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PMID: 3027506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation.

Molecular & general genetics : MGG ·Vol. 205 ·No. 2 ·1986-11-00 ·Pages 298-304

Irino N, Nakayama K, Nakayama H

Abstract

A 2,695 bp chromosomal segment of Escherichia coli K12 containing the recQ gene was sequenced. Analysis of the sequence revealed an open reading frame thought to represent recQ, with a clockwise direction of transcription relative to the standard genetic map of E. coli K12 and having a coding capacity for a protein of Mr 68,350. The -10 region of the presumptive recQ promoter overlapped the putative terminator for the upstream gene pldA, and was immediately followed by a 15 bp stretch of DNA bearing a strong resemblance to the reported sequences of LexA repressor binding sites. This latter finding suggested the possibility of SOS regulation of recQ gene expression, which was substantiated by experiments with recQ-lacZ fusions.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Codon DNA Repair DNA Transposable Elements Escherichia coli/genetics Genes Genes, Bacterial Plasmids SOS Response, Genetics Species Specificity
Chemicals
Bacterial Proteins Codon DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Irino N
Nakayama K
Nakayama H
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00430442
Published
1986-11-00
Pages
298-304
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00430442
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