A novel chlorophyll a/b binding (Cab) protein gene from petunia which encodes the lower molecular weight Cab precursor protein.

Nucleic acids research ·Vol. 14 ·No. 24 ·1986-12-22 ·Pages 9781-96

Stayton MM, Black M, Bedbrook J, Dunsmuir P

Abstract

The 16 petunia Cab genes which have been characterized are all closely related at the nucleotide sequence level and they encode Cab precursor polypeptides which are similar in sequence and length. Here we describe a novel petunia Cab gene which encodes a unique Cab precursor protein. This protein is a member of the smallest class of Cab precursor proteins for which no gene has previously been assigned in petunia or any other species. The features of this Cab precursor protein are that it is shorter by 2-3 amino acids than the formerly characterized Cab precursors, its transit peptide sequence is unrelated, and the mature polypeptide is significantly diverged at the functionally important N terminus from other petunia Cab proteins. Gene structure also discriminates this gene which is the only intron containing Cab gene in petunia genomic DNA.

MeSH Terms

Amino Acid Sequence Base Sequence Chlorophyll/genetics Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Light-Harvesting Protein Complexes Molecular Weight Nucleic Acid Hybridization Photosynthetic Reaction Center Complex Proteins Plant Proteins/genetics Plants/genetics Protein Biosynthesis Protein Precursors/genetics RNA, Messenger/genetics,isolation & purification Triticum/metabolism

Chemicals

Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Plant Proteins Protein Precursors RNA, Messenger Chlorophyll DNA DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Stayton M M

Black M

Bedbrook J

Dunsmuir P

References (18)

18 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.24.9781

Published

1986-12-22

Pages

9781-96

Language

English

Region

England

NLM ID

0411011

PMCID

PMC341335

Subset

Databases

GENBANK

X04966

External Links

PubMed source DOI: 10.1093/nar/14.24.9781 PMC Full Text View journal details

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A novel chlorophyll a/b binding (Cab) protein gene from petunia which encodes the lower molecular weight Cab precursor protein.

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