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PMID: 3029683 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.

Nucleic acids research ·Vol. 15 ·No. 2 ·1987-01-26 ·Pages 465-75

Gilchrist CA, Denhardt DT

Abstract

The sequence of a 2.67-kilobase section of the Escherichia coli chromosome that contains the rep gene has been determined. This gene codes for a protein of predicted Mr 72,800, a DNA helicase, which is also a single-stranded DNA-dependent ATPase. The sequenced region contains an open reading frame of the correct length and orientation to encode the Rep protein. A secondary structure for the protein can be formulated from the amino acid sequence. We have compared both the primary and the secondary structures of Rep with other proteins and find the greatest homology between Rep and E. coli helicase II, the product of the uvrD gene.

MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Base Sequence DNA Helicases DNA Restriction Enzymes Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes Genes, Bacterial Molecular Weight Sequence Homology, Nucleic Acid
Chemicals
Escherichia coli Proteins rep protein, E coli DNA Restriction Enzymes Adenosine Triphosphatases UvrD protein, E coli DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilchrist C A
Denhardt D T
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.2.465
Published
1987-01-26
Pages
465-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340446
Subset
IM
Databases
GENBANK
X04794
External Links
PubMed source DOI: 10.1093/nar/15.2.465 PMC Full Text View journal details
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