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PMID: 3029725 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.

Nucleic acids research ·Vol. 15 ·No. 4 ·1987-02-25 ·Pages 1717-28

Baroin A, Prat A, Caron F

Abstract

In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.

MeSH Terms
Animals Base Sequence Cell Nucleus/ultrastructure Chromosomes/ultrastructure DNA/genetics,isolation & purification DNA Restriction Enzymes Genes Membrane Glycoproteins Membrane Proteins/genetics Paramecium/genetics,ultrastructure Protozoan Proteins
Chemicals
Membrane Glycoproteins Membrane Proteins Protozoan Proteins surface protein G, Paramecium DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baroin A
Prat A
Caron F
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.4.1717
Published
1987-02-25
Pages
1717-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340576
Subset
IM
Databases
GENBANK
X04871, X04873, X04875, X04877
External Links
PubMed source DOI: 10.1093/nar/15.4.1717 PMC Full Text View journal details
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