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PMID: 3030732 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The first exon of the c-myc proto-oncogene contains a novel positive control element.

The EMBO journal ·Vol. 5 ·No. 13 ·1986-12-20 ·Pages 3553-62

Yang JQ, Remmers EF, Marcu KB

Abstract

We have identified a positive modulator within the c-myc first exon downstream of the gene's transcription initiation sites, P1 and P2. We introduced myc-CAT (chloramphenicol acetyltransferase) hybrid genes into three cell lines (BJAB, COS and HeLa) and measured their expression by either CAT enzymatic activity, S1 nuclease protection or by a nuclear 'run-on' transcription assay. Removal of 46 bp from the 3' end of the first exon results in a decrease of myc-CAT expression and P2 activity. A 438-bp exon 1 segment, lacking the normal myc promoters, efficiently drives the expression of SV40 early promoters. We find that this first exon segment efficiently functions as a positive modulator only in its sense orientation, 3' of a nearby promoter. The positive effects of the myc first exon and the SV40 enhancer are complementary.

MeSH Terms
Animals Cell Line DNA Restriction Enzymes Endonucleases Enhancer Elements, Genetic Exons Genes, Viral Genetic Vectors Proto-Oncogenes Simian virus 40/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yang J Q
Remmers E F
Marcu K B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-20
Pages
3553-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167393
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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