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PMID: 3031023 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

IS2 activates the ilvA gene of Pseudomonas cepacia in Escherichia coli.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1777-9

Barsomian G, Lessie TG

Abstract

The ilvA gene of Pseudomonas cepacia was expressed poorly in Escherichia coli. Insertion of IS2 upstream of the cloned gene dramatically increased its transcription, resulting in an 85-fold increase in threonine dehydratase (deaminase) specific activity. The results confirm earlier reports that IS2 promotes efficient expression of foreign genes in E. coli.

MeSH Terms

Cloning, Molecular DNA Transposable Elements Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial Pseudomonas/enzymology,genetics Threonine Dehydratase/biosynthesis,genetics Transcription, Genetic

Chemicals

DNA Transposable Elements Threonine Dehydratase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Barsomian G

Lessie T G

References (16)

16 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.4.1777-1779.1987

Published

1987-04-00

Pages

1777-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212018

Subset

External Links

PubMed source DOI: 10.1128/jb.169.4.1777-1779.1987 PMC Full Text View journal details

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IS2 activates the ilvA gene of Pseudomonas cepacia in Escherichia coli.

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