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PMID: 3031468 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Phosphorylation of talin at tyrosine in Rous sarcoma virus-transformed cells.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 371-8

DeClue JE, Martin GS

Abstract

The cytoskeletal protein talin was found to undergo enhanced phosphorylation at tyrosine residues in chicken embryo fibroblasts following transformation by Rous sarcoma virus. An increase in the tyrosine phosphorylation of talin was also observed within 6 h in cells infected by the temperature-sensitive mutant tsNY68 after a shift from the nonpermissive to the permissive temperature. The overall extent of phosphorylation was 0.07 mol of phosphate per mol of talin and was not appreciably altered by transformation. In uninfected cells talin was shown to be phosphorylated at multiple sites by tryptic peptide mapping. Following transformation most of these sites remained phosphorylated, to the same or to a lesser extent, while novel, phosphotyrosine-containing phosphopeptides appeared. Talin was phosphorylated at tyrosine in cells infected by Rous sarcoma virus mutants which induce altered or partial transformation morphologies; thus the increased phosphorylation of talin at tyrosine occurred irrespective of the morphology induced. Transformation by Y73 also induced elevated levels of phosphotyrosine in talin, whereas transformation by the avian erythroblastosis and Fujinami sarcoma viruses did not.

MeSH Terms
Amino Acids/analysis Animals Avian Sarcoma Viruses/genetics Cell Transformation, Neoplastic Chick Embryo Cytoskeletal Proteins/metabolism Fibroblasts/metabolism Mutation Peptide Mapping Phosphorus Radioisotopes Phosphorylation Talin Trypsin Tyrosine/metabolism
Chemicals
Amino Acids Cytoskeletal Proteins Phosphorus Radioisotopes Talin Tyrosine Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeClue J E
Martin G S
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57 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.371-378.1987
Published
1987-01-00
Pages
371-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365078
Subset
IM
Grants
PHS HHS · 17542 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.1.371-378.1987 PMC Full Text View journal details
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