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PMID: 3031607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity of a Streptomyces transcriptional terminator in Escherichia coli.

Nucleic acids research ·Vol. 15 ·No. 6 ·1987-03-25 ·Pages 2665-75

Deng ZX, Kieser T, Hopwood DA

Abstract

A 205bp DNA fragment from the Streptomyces multi-copy plasmid pIJ101 has in vivo terminator activity both in Streptomyces lividans and in Escherichia coli. Termination of RNA synthesis, detected by high-resolution S1 nuclease mapping, occurs at precisely the same nucleotides in both organisms. This suggests that the E. coli RNA polymerase recognizes the same sequence elements and chooses the point(s) of termination in the same way as the corresponding S. lividans enzyme.

MeSH Terms
Chloramphenicol/pharmacology DNA-Directed RNA Polymerases/metabolism Drug Resistance, Microbial Endonucleases Escherichia coli/enzymology,genetics Genes, Bacterial Genes, Regulator Kanamycin/pharmacology Single-Strand Specific DNA and RNA Endonucleases Streptomyces/drug effects,genetics Terminator Regions, Genetic Transcription, Genetic
Chemicals
Kanamycin Chloramphenicol DNA-Directed RNA Polymerases Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deng Z X
Kieser T
Hopwood D A
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.6.2665
Published
1987-03-25
Pages
2665-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340676
Subset
IM
Databases
GENBANK
X05172
External Links
PubMed source DOI: 10.1093/nar/15.6.2665 PMC Full Text View journal details
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