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PMID: 3031680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of a cDNA clone and localization of human glutathione S-transferase 2 genes to chromosome band 6p12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 8 ·1987-04-00 ·Pages 2377-81

Board PG, Webb GC

Abstract

The glutathione S-transferases (GST) (glutathione transferase; EC 2.5.1.18) are a family of enzymes responsible for the metabolism of a broad range of xenobiotics and carcinogens. A cDNA clone containing the entire amino acid coding sequence of a human GST-2 subunit has been isolated using a lambda gt11 expression library. The complete nucleotide sequence and a partial restriction map are presented. The subunit is composed of 221 amino acids with a molecular weight of 25,425 before posttranslational modification. The deduced amino acid sequence is rich in lysine, which is consistent with the relatively high pI of GST-2. The human sequence shows considerable homology with the rat Ya and Yc GST sequences but little homology with the rat GSTp and Yb subunit sequences. Southern blots of restriction digests of human DNA indicate that there may be multiple GST-2 genes. In situ hybridization of the cloned cDNA to human chromosomes produces intense labeling only over band p12 on the short arm of chromosome 6 near the centromere. This indicates that the GST-2 gene(s) are located only at this site.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Human, Pair 6 Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Glutathione Transferase/genetics Humans Isoenzymes/genetics Liver/enzymology Macromolecular Substances
Chemicals
Isoenzymes Macromolecular Substances DNA Glutathione Transferase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Board P G
Webb G C
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.8.2377
Published
1987-04-00
Pages
2377-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304654
Subset
IM
Databases
GENBANK
M15872
External Links
PubMed source DOI: 10.1073/pnas.84.8.2377 PMC Full Text View journal details
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