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PMID: 3032913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of cobalamin biosynthetic operons in Salmonella typhimurium.

Journal of bacteriology ·Vol. 169 ·No. 5 ·1987-05-00 ·Pages 2251-8

Escalante-Semerena JC, Roth JR

Abstract

Transcription of cobalamin (cob) biosynthetic genes in Salmonella typhimurium is repressed by cobalamin and by molecular oxygen. These genes seem to be subject to catabolite repression, and they are maximally expressed under conditions of anaerobic respiration of glycerol-fumarate. A 215-fold increase in the expression of cob genes occurs when S. typhimurium shifts from aerobic growth on glucose to anaerobic respiration of glycerol-fumarate under strictly anoxic growth conditions. Exogenous cyclic AMP substantially stimulates the transcription of cob-lac fusions during aerobic growth. However, cyclic AMP is not absolutely required for the expression of the pathway, nor does it mediate the aerobic control. Cobalamin biosynthesis is not seen under aerobic growth conditions, even when transcription is stimulated by the addition of cyclic AMP. Hence, additional control mechanisms triggered by the presence of molecular oxygen must operate independently from transcription effects on the cob operons.

MeSH Terms
Anaerobiosis Carbon/metabolism Cyclic AMP/physiology Gene Expression Regulation Genes, Bacterial Operon Oxygen/physiology Salmonella typhimurium/genetics,growth & development,metabolism Transcription, Genetic Vitamin B 12/biosynthesis,physiology
Chemicals
Carbon Cyclic AMP Vitamin B 12 Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Escalante-Semerena J C
Roth J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.5.2251-2258.1987
Published
1987-05-00
Pages
2251-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212145
Subset
IM
Grants
NIGMS NIH HHS · GM-34804 · United States
External Links
PubMed source DOI: 10.1128/jb.169.5.2251-2258.1987 PMC Full Text View journal details
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