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PMID: 3033633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and DNA sequence of the mercuric- and organomercurial-resistance determinants of plasmid pDU1358.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 10 ·1987-05-00 ·Pages 3112-6

Griffin HG, Foster TJ, Silver S, Misra TK

Abstract

The broad-spectrum mercurial-resistance plasmid pDU1358 was analyzed by cloning the resistance determinants and preparing a physical and genetic map of a 45-kilobase (kb) region of the plasmid that contains two separate mercurial-resistance operons that mapped about 20 kb apart. One encoded narrow-spectrum mercurial resistance to Hg2+ and a few organomercurials; the other specified broad-spectrum resistance to phenylmercury and additional organomercurials. Each determinant governed mercurial transport functions. Southern DNA X DNA hybridization experiments using gene-specific probes from the plasmid R100 mer operon indicated close homology with the R100 determinant. The 2153 base pairs of the promoter-distal part of the broad-spectrum Hg2+-resistance operon of pDU1358 were sequenced. This region included the 3'-terminal part of the merA gene, merD, unidentified reading frame URF1, and a part of URF2 homologous to previously sequenced determinants of plasmid R100. Between the merA and merD genes, an open reading frame encoding a 212 amino acid polypeptide was identified as the merB gene that determines the enzyme organomercurial lyase that cleaves the C--Hg bond of phenylmercury.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli/drug effects,genetics,growth & development Genes Genes, Bacterial Lyases/genetics Mercury/pharmacology Nucleic Acid Hybridization Organomercury Compounds/pharmacology R Factors beta-Galactosidase/genetics,metabolism
Chemicals
Organomercury Compounds DNA Restriction Enzymes beta-Galactosidase Lyases alkylmercury lyase Mercury
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Griffin H G
Foster T J
Silver S
Misra T K
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.10.3112
Published
1987-05-00
Pages
3112-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304818
Subset
IM
Grants
NIAID NIH HHS · AI15672 · United States
Databases
GENBANK
M15049
External Links
PubMed source DOI: 10.1073/pnas.84.10.3112 PMC Full Text View journal details
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