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PMID: 3034861 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2639-42

Sung YC, Parsell D, Anderson PM, Fuchs JA

Abstract

The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.

MeSH Terms
Aminohydrolases/genetics Bacterial Proteins/genetics Carbon-Nitrogen Lyases Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial
Chemicals
Bacterial Proteins DNA Restriction Enzymes Aminohydrolases cyanate hydrolase Carbon-Nitrogen Lyases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sung Y C
Parsell D
Anderson P M
Fuchs J A
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2639-2642.1987
Published
1987-06-00
Pages
2639-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212143
Subset
IM
Grants
NIGMS NIH HHS · GM 33842 · United States
NCRR NIH HHS · SO7RR05896 · United States
External Links
PubMed source DOI: 10.1128/jb.169.6.2639-2642.1987 PMC Full Text View journal details
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