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PMID: 3034916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures.

The Journal of cell biology ·Vol. 104 ·No. 6 ·1987-06-00 ·Pages 1521-6

Stickel SK, Wang YL

Abstract

Normal rat kidney cells infected with a Rous sarcoma virus (strain LA23) were used to study the dynamics of alpha-actinin-containing aggregates in transformed cells. Experiments were performed by microinjecting living cells with iodoacetamidotetramethylrhodamine alpha-actinin and allowing the fluorescent analogue to incorporate into cellular structures. Subsequent time-lapse recording indicated that the alpha-actinin-containing aggregates can undergo rapid formation, movement, and breakdown. In addition, experiments using the photobleaching recovery technique indicated that alpha-actinin molecules associated with the aggregates have a very high rate of exchange, whereas those associated with adhesion plaques in normal cells exchange much more slowly. The dynamic properties of alpha-actinin-containing aggregates may be closely related to the changes in cellular behavior upon oncogenic transformation.

MeSH Terms
Actinin/metabolism Animals Avian Sarcoma Viruses/physiology Cell Adhesion Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Microscopy, Fluorescence Rats
Chemicals
Actinin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stickel S K
Wang Y L
References (18)
18 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.104.6.1521
Published
1987-06-00
Pages
1521-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114522
Subset
IM
Grants
NIGMS NIH HHS · GM-32476 · United States
External Links
PubMed source DOI: 10.1083/jcb.104.6.1521 PMC Full Text View journal details
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