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PMID: 3035494 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cosmid mapping of the human chorionic gonadotropin beta subunit genes by field-inversion gel electrophoresis.

Nucleic acids research ·Vol. 15 ·No. 11 ·1987-06-11 ·Pages 4437-48

Graham MY, Otani T, Boime I, Olson MV, Carle GF, Chaplin DD

Abstract

A cosmid clone containing the entire hCG beta gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCG beta genes in human genomic DNA. A previously uncloned portion of the hCG beta cluster, termed the "gap" region, has been shown not to contain any sequences homologous to the hCG beta cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.

MeSH Terms
Chorionic Gonadotropin/genetics Chromosome Mapping Cosmids DNA Restriction Enzymes Electrophoresis, Agar Gel/methods Genetic Linkage Humans Multigene Family Nucleic Acid Hybridization
Chemicals
Chorionic Gonadotropin DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Graham M Y
Otani T
Boime I
Olson M V
Carle G F
Chaplin D D
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.11.4437
Published
1987-06-11
Pages
4437-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340872
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.11.4437 PMC Full Text View journal details
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