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PMID: 3036490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria.

The EMBO journal ·Vol. 6 ·No. 4 ·1987-04-00 ·Pages 1073-7

Eilers M, Oppliger W, Schatz G

Abstract

We have investigated the energy requirement of mitochondrial protein import with a simplified system containing only isolated yeast mitochondria, energy sources and a purified precursor protein. This precursor was a fusion protein composed of 22 residues of the cytochrome oxidase subunit IV pre-sequence fused to mouse dihydrofolate reductase. Import of this protein required not only an energized inner membrane, but also ATP. ATP could be replaced by GTP, but not by CTP, TTP or non-hydrolyzable ATP analogs. Added ATP did not increase the membrane potential of respiring mitochondria; it supported import even if the proton-translocating mitochondrial ATPase and the entry of ATP into the matrix were blocked. We conclude that ATP exerts its effect on mitochondrial protein import outside the inner membrane.

MeSH Terms
Adenosine Triphosphate/metabolism Biological Transport Electron Transport Complex IV/genetics,metabolism Intracellular Membranes/metabolism Macromolecular Substances Membrane Potentials Mitochondria/metabolism Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism Ribonucleotides/metabolism Saccharomyces cerevisiae/metabolism Tetrahydrofolate Dehydrogenase/genetics,metabolism
Chemicals
Macromolecular Substances Recombinant Fusion Proteins Recombinant Proteins Ribonucleotides Adenosine Triphosphate Tetrahydrofolate Dehydrogenase Electron Transport Complex IV
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eilers M
Oppliger W
Schatz G
References (27)
27 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-04-00
Pages
1073-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553504
Subset
IM
Grants
NIGMS NIH HHS · 1 RO1 GM37803-01 · United States
External Links
PubMed source PMC Full Text View journal details
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