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PMID: 3036778 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cloning of the cyo locus encoding the cytochrome o terminal oxidase complex of Escherichia coli.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 3237-42

Au DC, Gennis RB

Abstract

The structural genes encoding the cytochrome o terminal oxidase complex (cyo) of Escherichia coli have been subcloned into the multicopy plasmid pBR322 after the Mu-mediated transposition of the gene locus from the bacterial chromosome onto the conjugative R plasmid RP4. Introduction of cyo plasmids into strains (cyo cyd) lacking both terminal oxidases restored the ability of the strains to grow aerobically on nonfermentable substrates. Strains carrying the cyo plasmids produced 5 to 10 times more cytochrome o oxidase than did control strains. The gene products encoded by the cyo plasmids could be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cloned genes will be valuable for studying the structure, function, and regulation of the cytochrome o terminal oxidase complex.

MeSH Terms
Bacteriophage mu/genetics Chromosome Mapping Chromosomes, Bacterial/ultrastructure Cloning, Molecular DNA, Bacterial/genetics Electron Transport Complex IV/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Multienzyme Complexes Mutation Plasmids
Chemicals
DNA, Bacterial Multienzyme Complexes Electron Transport Complex IV
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Au D C
Gennis R B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.7.3237-3242.1987
Published
1987-07-00
Pages
3237-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212375
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.7.3237-3242.1987 PMC Full Text View journal details
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