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PMID: 3037541 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Spermatid-specific expression of protamine 1 in transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 15 ·1987-08-00 ·Pages 5316-9

Peschon JJ, Behringer RR, Brinster RL, Palmiter RD

Abstract

Protamines are abundant basic proteins involved in the condensation of sperm chromatin. In the mouse, protamine genes are transcribed postmeiotically in round spermatids. We have cloned and sequenced the mouse protamine 1 gene. Ten lines of transgenic mice harboring marked protamine 1 sequences were generated by microinjection of fertilized eggs. Transcription of the transgene is restricted to round spermatids and in several cases exceeds that of the endogenous gene. The cis-acting sequences required for tissue-specific protamine expression reside on a 2.4-kilobase restriction fragment. Prospects for using transgenic mice to address fundamental questions of male germ-cell development are discussed.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes/metabolism Gene Expression Regulation Male Mice Mice, Inbred C57BL Microinjections Nucleic Acid Hybridization Protamines/genetics Spermatids/metabolism Tissue Distribution
Chemicals
Protamines DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peschon J J
Behringer R R
Brinster R L
Palmiter R D
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.15.5316
Published
1987-08-00
Pages
5316-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298846
Subset
IM
Grants
NICHD NIH HHS · HD09172 · United States
NICHD NIH HHS · HD17321 · United States
Databases
GENBANK
M17217
External Links
PubMed source DOI: 10.1073/pnas.84.15.5316 PMC Full Text View journal details
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