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PMID: 3038517 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two different factors bind to the alpha-domain of the polyoma virus enhancer, one of which also interacts with the SV40 and c-fos enhancers.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1331-7

Piette J, Yaniv M

Abstract

Two nuclear factors from mouse 3T6 cells bind to a 22-bp segment constituting the alpha-domain of the polyoma virus enhancer. Binding of each factor can be competed out selectively by the appropriate double-stranded oligonucleotide, indicating that this binding is not strictly cooperative. Sequence homology between the two binding sites and the similar size of the protected regions may indicate that both factors, PEA1 and PEA2, are closely related. The binding site of PEA1 is centered on a sequence showing strong homology to the SV40 enhancer, the binding site of PEA2 is located immediately adjacent to it and shows a strong homology to the c-fos enhancer. Surprisingly, both SV40 and c-fos enhancers interact with PEA1, probably due to the presence of an extra base pair relative to c-fos in the PEA2 site. Factor PEA1 is probably identical to the recently described activator protein 1 (AP1).

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Cells, Cultured DNA Restriction Enzymes Enhancer Elements, Genetic Genes, Regulator Genes, Viral Mice Polyomavirus/genetics Proto-Oncogenes Sequence Homology, Nucleic Acid Simian virus 40/genetics
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Piette J
Yaniv M
References (36)
36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1331-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553937
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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