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PMID: 3039176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus 1 protein kinase is encoded by open reading frame US3 which is not essential for virus growth in cell culture.

Journal of virology ·Vol. 61 ·No. 9 ·1987-09-00 ·Pages 2896-901

Purves FC, Longnecker RM, Leader DP, Roizman B

Abstract

Earlier reports have described a novel protein kinase in cells infected with herpes simplex or pseudorabies viruses. These novel enzymes were characterized by their acceptance of protamine as a substrate and by their differential chromatographic behavior in anion-exchange chromatography. We report that this activity was not present in extracts of uninfected cells or of cells infected with a mutant constructed so as to contain a deletion in the US3 open reading frame mapping in the small component of herpes simplex virus 1 DNA. The activity was present in extracts of cells infected with wild-type virus and with a recombinant in which the US3 open reading frame had been rescued. Our results are consistent with the observation reported earlier that the coding sequences predict an amino acid motif common to protein kinases and lead to the conclusion that the US3 open reading frame encodes a virus-specific protein kinase that is not required for virus growth in cells in culture.

MeSH Terms
Amino Acid Sequence Cells, Cultured Genes, Viral Mutation Protein Kinases/analysis,genetics Simplexvirus/enzymology,growth & development Thymidine Kinase/genetics Transfection
Chemicals
Protein Kinases Thymidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Purves F C
Longnecker R M
Leader D P
Roizman B
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.9.2896-2901.1987
Published
1987-09-00
Pages
2896-901
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255814
Subset
IM
Grants
NCI NIH HHS · CA-08494 · United States
NCI NIH HHS · CA-19264 · United States
NIAID NIH HHS · T32 PHS AI 07182 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.9.2896-2901.1987 PMC Full Text View journal details
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