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PMID: 3039294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the pyrimidine specific carbamoyl phosphate synthetase, a part of the yeast multifunctional protein encoded by the URA2 gene.

Molecular & general genetics : MGG ·Vol. 207 ·No. 2-3 ·1987-05-00 ·Pages 314-9

Souciet JL, Potier S, Hubert JC, Lacroute F

Abstract

Yeast URA2 encodes a multifunctional carbamoyl phosphate synthetase-aspartate transcarbamylase of 220,000 molecular weight. We determined the nucleotide sequence of the 5' proximal part of the gene which is responsible for the glutamine amide transfer function of the carbamoyl phosphate synthetase activity. Alignment of the enzyme sequence derived from URA2 with sequences from Escherichia coli carA carB and yeast arginine-specific CP A1 CP A2 indicates that monofunctional and bifunctional carbamoyl phosphate synthetases are probably homologous. The URA2-derived enzyme organization is NH2-carbamoyl phosphate synthetase-aspartate transcarbamylase-CO2H.

MeSH Terms
Amino Acid Sequence Aspartate Carbamoyltransferase/genetics Base Sequence Biological Evolution Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics DNA Restriction Enzymes Genes Genes, Fungal Ligases/genetics Multienzyme Complexes/genetics Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Multienzyme Complexes Aspartate Carbamoyltransferase DNA Restriction Enzymes Ligases Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Souciet J L
Potier S
Hubert J C
Lacroute F
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331595
Published
1987-05-00
Pages
314-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X05553
External Links
PubMed source DOI: 10.1007/BF00331595
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