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PMID: 3039462 Published · ppublish English Journal Article

Putative promoter elements for the ribosomal RNA genes of the thermoacidophilic archaebacterium Sulfolobus sp. strain B12.

Nucleic acids research ·Vol. 15 ·No. 14 ·1987-07-24 ·Pages 5581-95

Reiter WD, Palm P, Voos W, Kaniecki J, Grampp B, Schulz W, Zillig W

Abstract

In Sulfolobus sp. strain B12, single-copy genes encode the three ribosomal RNAs. The genes for the 16S rRNA and for the 23S rRNA are closely linked but separated from the 5S rRNA gene. Transcription of the 16S/23S rRNA gene cluster starts 139 nucleotides upstream of the 5'-end of mature 16S rRNA. For the 5S rRNA gene the point of transcription initiation coincides with the 5'-end of mature 5S rRNA. The comparison of the upstream regions for these transcriptional start sites shows the presence of a completely conserved trinucleotide sequence around the point of transcription initiation and a completely conserved octanucleotide sequence about 22 nucleotides upstream of it. These sequences are only moderately homologous to putative promoter elements for stable RNA genes in the closely related archaebacterium Thermoproteus tenax (1), but they are very similar to corresponding sequences in the distantly related archaebacterium Methanococcus vannielii (2). The consensus sequence found for Sulfolobus and Methanococcus could therefore constitute the archetype of an archaebacterial promoter for stable RNA genes.

MeSH Terms
Archaea/genetics Bacteria/genetics Base Sequence DNA Restriction Enzymes Euryarchaeota/genetics Genes, Bacterial Promoter Regions, Genetic RNA, Ribosomal/genetics Species Specificity
Chemicals
RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Reiter W D
Palm P
Voos W
Kaniecki J
Grampp B
Schulz W
Zillig W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.14.5581
Published
1987-07-24
Pages
5581-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306008
Subset
IM
Databases
GENBANK
X05869, X05870
External Links
PubMed source DOI: 10.1093/nar/15.14.5581 PMC Full Text View journal details
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