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PMID: 3040685 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence analysis of the gtfB gene from Streptococcus mutans.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 4263-70

Shiroza T, Ueda S, Kuramitsu HK

Abstract

The nucleotide sequence of the gtfB gene from Streptococcus mutans GS-5, coding for glucosyltransferase I activity, was determined. The gene codes for a strongly hydrophilic protein with a molecular size of 165,800 daltons. The deduced amino acid sequence revealed a typical gram-positive bacterial signal sequence at the NH2 terminus of the protein and 3.5 direct repeating units (each containing 65 amino acids) at the COOH terminus. Nucleotide sequencing of the region immediately downstream from the gtfB gene revealed the presence of a putative gene coding for an extracellular protein. This open reading frame is partially homologous to the gtfB gene.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes DNA, Bacterial/analysis Genes, Bacterial Glucosyltransferases/genetics Sequence Homology, Nucleic Acid Streptococcus mutans/enzymology,genetics
Chemicals
DNA, Bacterial 1,3-alpha-D-glucan synthase Glucosyltransferases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shiroza T
Ueda S
Kuramitsu H K
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.9.4263-4270.1987
Published
1987-09-00
Pages
4263-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213739
Subset
IM
Grants
NIDCR NIH HHS · DE-03258 · United States
NIDCR NIH HHS · DE-06080 · United States
Databases
GENBANK
M17361
External Links
PubMed source DOI: 10.1128/jb.169.9.4263-4270.1987 PMC Full Text View journal details
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