Home LiteratureArticle Details
PMID: 3040693 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the DNA sequence required for transposition immunity of the gamma delta sequence.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 4388-90

Goto N, Mochizuki A, Inagaki Y, Horiuchi S, Tanaka T, Nakaya R

Abstract

A plasmid containing the transposon gamma delta sequence was immune to further insertion of gamma delta (transposition immunity). Plasmids carrying a fragment containing either 0.2 kilobase pairs of the gamma end or 0.4 kilobase pairs of the delta end of the gamma delta sequence were immune, and other parts of the gamma delta sequence did not confer immunity. The terminal 38-base-pair (bp) sequence of the delta end of the gamma delta was sufficient to confer immunity, the 38-bp sequence of the gamma end conferred only moderate immunity, and the terminal 35-bp sequence, which was completely identical at both the gamma and delta ends, was insufficient to confer immunity.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/analysis,genetics Genes, Bacterial Plasmids
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Goto N
Mochizuki A
Inagaki Y
Horiuchi S
Tanaka T
Nakaya R
References (12)
12 references, click to expand
  1. Inhibition of TnA translocation by TnA.
    J Bacteriol. 1977 Jan;129(1):407-14 PMID: 318647
  2. Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa.
    J Bacteriol. 1977 Mar;129(3):1227-33 PMID: 403173
  3. The gamma delta sequence of F is an insertion sequence.
    J Mol Biol. 1978 Dec 15;126(3):347-65 PMID: 745233
  4. Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification.
    Mol Gen Genet. 1979 Apr 17;172(1):53-65 PMID: 377024
  5. Transposition of the Escherichia coli insertion element gamma generates a five-base-pair repeat.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4882-6 PMID: 388421
  6. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  7. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428-32 PMID: 6267590
  8. The location of sequences of TnA required for the establishment of transposition immunity.
    Mol Gen Genet. 1981;184(1):80-6 PMID: 6278249
  9. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  10. Identification of a transposon Tn3 sequence required for transposition immunity.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6765-9 PMID: 6316324
  11. Conduction of nonconjugative plasmids by F' lac is not necessarily associated with transposition of the gamma delta sequence.
    J Bacteriol. 1984 Aug;159(2):590-6 PMID: 6086578
  12. Analysis of Tn3 sequences required for transposition and immunity.
    Gene. 1986;41(1):23-31 PMID: 3009272
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1987-09-00
Pages
4388-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213758
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]