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PMID: 304074 Published · ppublish English Journal Article

Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes.

Journal of immunological methods ·Vol. 18 ·No. 3-4 ·1977-00-00 ·Pages 387-9

Zola H

Abstract

Human B lymphocytes from rosettes with mouse erythrocytes. If the erythrocytes are pretreated with papain the rosettes are sufficiently stable to allow separation of T and B lymphocytes. The technique is complementary to the sheep erythrocyte rosette method, and gives cell populations with a similar degree of purity.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Separation Erythrocytes/immunology Humans Mice Papain/pharmacology Receptors, Antigen, B-Cell/metabolism Rosette Formation T-Lymphocytes/immunology
Chemicals
Receptors, Antigen, B-Cell Papain
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Zola H
Article Info
Journal
Journal of immunological methods
Abbr.
J Immunol Methods
ISSN
0022-1759
Published
1977-00-00
Pages
387-9
Language
English
Region
Netherlands
NLM ID
1305440
Subset
IM
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