Home LiteratureArticle Details
PMID: 3041033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CD8-positive T lymphocytes specific for murine cytomegalovirus immediate-early antigens mediate protective immunity.

Journal of virology ·Vol. 61 ·No. 10 ·1987-10-00 ·Pages 3102-8

Reddehase MJ, Mutter W, Münch K, Bühring HJ, Koszinowski UH

Abstract

We have shown in a murine model system for acute, lethal cytomegalovirus (CMV) disease in the immunocompromised natural host that control of virus multiplication in tissues, protection from virus-caused tissue destruction, and survival are mediated by virus-specific CD8+ CD4-T lymphocytes. Protection from a lethal course of disease did not result in a rapid establishment of virus latency, but led to a long-lasting, persistent state of infection. The CD8- CD4+ subset of T lymphocytes was not effective by itself in controlling murine CMV (MCMV) multiplication in tissue or essential for the protective function of the CD8+ CD4- effector cells. The antiviral efficacy of the purified CD8+ CD4- subset was not impaired by preincubation with fibroblasts that presented viral structural antigens, but was significantly reduced after depletion of effector cells specific for the nonstructural immediate-early antigens of MCMV, which are specified by the first among a multitude of viral genes expressed during MCMV replication in permissive cells. Thus, MCMV disease provides the first example of a role for nonstructural herpesvirus immediate-early antigens in protective immunity.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface Antigens, Viral/genetics,immunology Cell Line Cloning, Molecular Cytomegalovirus/genetics,immunology,physiology Cytomegalovirus Infections/immunology Fibroblasts Genes, Viral Immediate-Early Proteins Immune Tolerance Immunity, Cellular Mice Mice, Inbred BALB C Nucleic Acid Hybridization Phenotype T-Lymphocytes/immunology Virus Replication
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface Antigens, Viral Immediate-Early Proteins immediate-early proteins, cytomegalovirus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reddehase M J
Mutter W
Münch K
Bühring H J
Koszinowski U H
References (39)
39 references, click to expand
  1. Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.
    J Virol. 1974 Jul;14(1):8-19 PMID: 4365321
  2. Reactivation of murine cytomegalovirus by cyclophosphamide.
    Nature. 1977 Jun 23;267(5613):721-3 PMID: 195225
  3. Genetic control of the cytotoxic T cell response to SV40 tumor-associated specific antigen.
    J Immunol. 1979 May;122(5):1798-806 PMID: 87443
  4. Qat-4 and Qat-5, new murine T-cell antigens governed by the Tla region and identified by monoclonal antibodies.
    J Exp Med. 1979 Jul 1;150(1):108-16 PMID: 312902
  5. Cytomegalovirus infection and specific cell-mediated immunity after marrow transplant.
    J Infect Dis. 1980 Dec;142(6):816-24 PMID: 6257800
  6. The evaluation of limiting dilution assays.
    J Immunol Methods. 1982 Mar 12;49(2):R11-23 PMID: 7040548
  7. Cytotoxic t cells in cytomegalovirus infection: HLA-restricted T-lymphocyte and non-T-lymphocyte cytotoxic responses correlate with recovery from cytomegalovirus infection in bone-marrow-transplant recipients.
    N Engl J Med. 1982 Jul 1;307(1):7-13 PMID: 6281647
  8. Demonstration of multiple antigenic sites of the SV40 transplantation rejection antigen by using cytotoxic T lymphocyte clones.
    J Immunol. 1983 Jan;130(1):490-2 PMID: 6183360
  9. Molecular cloning and physical mapping of murine cytomegalovirus DNA.
    J Virol. 1983 Sep;47(3):421-33 PMID: 6312075
  10. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity.
    Immunol Rev. 1983;74:29-56 PMID: 6195085
  11. Recognition by cytotoxic T lymphocytes of cells expressing fragments of the SV40 tumor antigen.
    J Immunol. 1983 Nov;131(5):2580-6 PMID: 6313808
  12. Human cytomegalovirus-specific cytotoxic T lymphocytes: requirements for in vitro generation and specificity.
    Eur J Immunol. 1983 Oct;13(10):804-9 PMID: 6196203
  13. The cytolytic T lymphocyte response to the murine cytomegalovirus. I. Distinct maturation stages of cytolytic T lymphocytes constitute the cellular immune response during acute infection of mice with the murine cytomegalovirus.
    J Immunol. 1984 Jan;132(1):482-9 PMID: 6317749
  14. The cytolytic T lymphocyte response to the murine cytomegalovirus. II. Detection of virus replication stage-specific antigens by separate populations of in vivo active cytolytic T lymphocyte precursors.
    Eur J Immunol. 1984 Jan;14(1):56-61 PMID: 6319154
  15. Temporal regulation of murine cytomegalovirus transcription and mapping of viral RNA synthesized at immediate early times after infection.
    J Virol. 1984 Jun;50(3):784-95 PMID: 6328008
  16. In vivo effector function of influenza virus-specific cytotoxic T lymphocyte clones is highly specific.
    J Exp Med. 1984 Sep 1;160(3):814-26 PMID: 6206190
  17. Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):6134-8 PMID: 6091137
  18. Significance of herpesvirus immediate early gene expression in cellular immunity to cytomegalovirus infection.
    Nature. 1984 Nov 22-28;312(5992):369-71 PMID: 6095095
  19. Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1785-9 PMID: 3872457
  20. Characterization of the major immediate-early polypeptides encoded by murine cytomegalovirus.
    J Virol. 1985 May;54(2):422-8 PMID: 2985805
  21. Acquired immunodeficiency syndrome. Clinicopathologic study of 56 autopsies.
    Arch Pathol Lab Med. 1985 Aug;109(8):727-34 PMID: 2990378
  22. Immediate causes of death in acquired immunodeficiency syndrome.
    Arch Pathol Lab Med. 1985 Aug;109(8):735-8 PMID: 2990379
  23. Interstitial murine cytomegalovirus pneumonia after irradiation: characterization of cells that limit viral replication during established infection of the lungs.
    J Virol. 1985 Aug;55(2):264-73 PMID: 2991554
  24. Cytotoxic T cells recognize fragments of the influenza nucleoprotein.
    Cell. 1985 Sep;42(2):457-67 PMID: 2411422
  25. Cloned long-term cytolytic T-lymphocyte line with specificity for an immediate-early membrane antigen of murine cytomegalovirus.
    J Virol. 1986 Jan;57(1):408-12 PMID: 3001363
  26. The 89,000-Mr murine cytomegalovirus immediate-early protein activates gene transcription.
    J Virol. 1986 Apr;58(1):59-66 PMID: 3005659
  27. Murine cytomegalovirus adrenalitis in athymic nude mice.
    Arch Virol. 1986;88(1-2):27-35 PMID: 3006642
  28. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.
    Cell. 1986 Mar 28;44(6):959-68 PMID: 2420472
  29. Cancer immunotherapy using interleukin-2 and interleukin-2-activated lymphocytes.
    Annu Rev Immunol. 1986;4:681-709 PMID: 3518753
  30. Recognition of cloned vesicular stomatitis virus internal and external gene products by cytotoxic T lymphocytes.
    J Exp Med. 1986 Jun 1;163(6):1529-38 PMID: 3011949
  31. Cytoimmunotherapy for persistent virus infection reveals a unique clearance pattern from the central nervous system.
    Nature. 1986 May 15-21;321(6067):239-43 PMID: 3086743
  32. T cell-mediated hepatitis in mice infected with lymphocytic choriomeningitis virus. Liver cell destruction by H-2 class I-restricted virus-specific cytotoxic T cells as a physiological correlate of the 51Cr-release assay?
    J Exp Med. 1986 Oct 1;164(4):1075-92 PMID: 3489805
  33. A nonstructural polypeptide encoded by immediate-early transcription unit 1 of murine cytomegalovirus is recognized by cytolytic T lymphocytes.
    J Exp Med. 1987 Jul 1;166(1):289-94 PMID: 2439637
  34. Late-phase expression of a murine cytomegalovirus immediate-early antigen recognized by cytolytic T lymphocytes.
    J Virol. 1986 Dec;60(3):1125-9 PMID: 2431160
  35. Modification of acute murine cytomegalovirus adrenal gland infection by adoptive spleen cell transfer.
    J Virol. 1987 Jan;61(1):23-8 PMID: 3023703
  36. Immediate-early genes of murine cytomegalovirus: location, transcripts, and translation products.
    J Virol. 1987 Feb;61(2):526-33 PMID: 3027381
  37. In vivo application of recombinant interleukin 2 in the immunotherapy of established cytomegalovirus infection.
    J Exp Med. 1987 Mar 1;165(3):650-6 PMID: 3029272
  38. Sequence and structural organization of murine cytomegalovirus immediate-early gene 1.
    J Virol. 1987 Jun;61(6):1901-8 PMID: 3033321
  39. Host immune response to cytomegalovirus: products of transfected viral immediate-early genes are recognized by cloned cytolytic T lymphocytes.
    J Virol. 1987 Jun;61(6):2054-8 PMID: 3033334
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.10.3102-3108.1987
Published
1987-10-00
Pages
3102-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255886
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.10.3102-3108.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2