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PMID: 3043182 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a Ty1 regulatory sequence responsive to STE7 and STE12.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2545-54

Company M, Adler C, Errede B

Abstract

Ty1 activation of gene expression observed in haploid cell types of Saccharomyces cerevisiae requires the STE7 and STE12 gene products. An activator sequence within Ty1 that is responsive to these two regulators has been defined. Complex formation between a factor in whole-cell extracts and the DNA regulatory element showed the same dependence on the STE7 and STE12 gene products as did reporter gene expression. Base pair substitutions within the binding site abolished the ability to form the factor-DNA complex and to activate gene expression. The correlation between complex formation and reporter gene expression indicates that factor binding to the cis-acting element is essential for gene activation. Because the predicted protein for the STE7 gene product is homologous to protein kinases, we suggest that protein phosphorylation may directly or indirectly regulate formation of this DNA-protein complex.

MeSH Terms
Base Sequence DNA/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation Haploidy Methylation Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Transcription, Genetic Transcriptional Activation
Chemicals
DNA-Binding Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Company M
Department of Chemistry, University of North Carolina, Chapel Hill 27514.
Adler C
Errede B
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2545-2554.1988
Published
1988-06-00
Pages
2545-54
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363455
Subset
IM
Grants
NIGMS NIH HHS · GM30619 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.6.2545-2554.1988 PMC Full Text View journal details
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