Abstract
Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells (Wright, R. L., J. Salisbury, and J. Jarvik. 1985. J. Cell Biol. 101:1903-1912; Salisbury, J. L., M. A. Sanders, and L. Harpst. 1987. J. Cell Biol. 105:1799-1805). Immunofluorescence studies show dramatic changes in distribution of the centrin-based system during mitosis that include a transient contraction at preprophase; division, separation, and re-extension during prophase; and a second transient contraction at the metaphase/anaphase boundary. These observations suggest a fundamental role for centrin in motile events during mitosis.
MeSH Terms
Animals
Calcium-Binding Proteins
Chlamydomonas/chemistry,cytology,ultrastructure
Chromosomal Proteins, Non-Histone
Contractile Proteins/analysis
Cytoskeleton/chemistry,ultrastructure
Electrophoresis, Polyacrylamide Gel
Flagella/chemistry,ultrastructure
Fluorescent Antibody Technique
Immunoassay
Immunohistochemistry
Interphase
Microscopy, Electron
Mitosis
Chemicals
Calcium-Binding Proteins
Chromosomal Proteins, Non-Histone
Contractile Proteins
caltractin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Salisbury J L
Center for NeuroSciences, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Baron A T
Sanders M A
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