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PMID: 3054808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

Nucleic acids research ·Vol. 16 ·No. 20 ·1988-10-25 ·Pages 9545-55

Jeppesen C, Jensen KF, Nielsen PE

Abstract

Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.

MeSH Terms
Cross-Linking Reagents DNA/radiation effects DNA-Directed RNA Polymerases/genetics,radiation effects Escherichia coli/enzymology,genetics,radiation effects Lac Operon/radiation effects Photochemistry Promoter Regions, Genetic/radiation effects Templates, Genetic Thymine/radiation effects Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
Cross-Linking Reagents DNA DNA-Directed RNA Polymerases Thymine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jeppesen C
Department of Biochemistry B, University of Copenhagen, Denmark.
Jensen K F
Nielsen P E
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.20.9545
Published
1988-10-25
Pages
9545-55
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338762
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.20.9545 PMC Full Text View journal details
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