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PMID: 3054878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Growth inhibition of human breast carcinoma and leukemia/lymphoma cell lines by recombinant interferon-beta 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8037-41

Chen L, Mory Y, Zilberstein A, Revel M

Abstract

Recombinant human interferon-beta 2 was produced in Escherichia coli by direct expression of cDNA encoding the mature protein sequence. At concentrations that stimulate DNA synthesis and growth in B-cell hybridomas and plasmacytomas, the cytokine was found to exert a strong inhibition on the growth of a number of carcinoma and leukemia/lymphoma cell lines. This antigrowth effect was observed in clonogenic assays and by measurements of cell number and thymidine incorporation in growing cultures. The effect was blocked by antibodies to a synthetic peptide from the N terminus of the molecule. Normal diploid fibroblasts were inhibited at concentrations higher than those needed for breast carcinoma cells.

MeSH Terms
Breast Neoplasms/pathology Cell Division/drug effects Cell Line DNA/metabolism Escherichia coli/genetics,metabolism Humans Interferon Type I/pharmacology Leukemia/pathology Lymphoma/pathology Recombinant Proteins/pharmacology
Chemicals
Interferon Type I Recombinant Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chen L
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
Mory Y
Zilberstein A
Revel M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8037
Published
1988-11-00
Pages
8037-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282349
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.21.8037 PMC Full Text View journal details
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