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PMID: 3056926 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export.

Journal of bacteriology ·Vol. 170 ·No. 12 ·1988-12-00 ·Pages 5928-30

Trun NJ, Stader J, Lupas A, Kumamoto C, Silhavy TJ

Abstract

We exploited the conditional-lethal phenotype of secB null mutations to demonstrate that SecB function was required for PrlA-mediated suppression of signal sequence mutations. The results of these experiments provide information about the functions performed and the sequence determinants recognized by each of these components of the protein export machinery of Escherichia coli.

MeSH Terms
Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Escherichia coli/genetics,metabolism Escherichia coli Proteins Genes Genes, Bacterial Mutation Phenotype Porins Receptors, Virus/genetics SEC Translocation Channels
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Proteins Porins Receptors, Virus SEC Translocation Channels SecB protein, Bacteria SecY protein, E coli maltoporins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Trun N J
Department of Biology, Princeton University, New Jersey 08544.
Stader J
Lupas A
Kumamoto C
Silhavy T J
References (6)
6 references, click to expand
  1. Suppressor mutations that restore export of a protein with a defective signal sequence.
    Cell. 1981 Jan;23(1):79-88 PMID: 7011570
  2. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
    Nucleic Acids Res. 1983 May 11;11(9):2599-616 PMID: 6222285
  3. The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein.
    Cell. 1988 Apr 22;53(2):273-83 PMID: 2834066
  4. A signal sequence receptor in the endoplasmic reticulum membrane.
    Nature. 1987 Aug 27-Sep 2;328(6133):830-3 PMID: 3041222
  5. Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7448-52 PMID: 2823262
  6. Evidence for specificity at an early step in protein export in Escherichia coli.
    J Bacteriol. 1985 Jul;163(1):267-74 PMID: 3891730
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.12.5928-5930.1988
Published
1988-12-00
Pages
5928-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211706
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.12.5928-5930.1988 PMC Full Text View journal details
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