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PMID: 3060848 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Exploration of the L18 binding site on 5S RNA by deletion mutagenesis.

Nucleic acids research ·Vol. 16 ·No. 22 ·1988-11-25 ·Pages 10717-32

Gewirth DT, Moore PB

Abstract

Several deletion variants of E. coli 5S RNA have been constructed and produced either in vivo or in vitro using T7 RNA Polymerase. Their structures and ribosomal protein L18 binding properties have been examined. All of them are similar to wild-type 5S RNA in their helix II-III regions, where L18 binds [Huber, P.W. and Wool, I.G. (1984) Proc. Natl. Acad. Sci. (USA) 81, 322-326; Douthwaite, S., Christensen, A., and Garrett, R.A. (1982) Biochemistry 21, 2313-2320.], by NMR criteria. However, none of the molecules examined that lack the helix IV-helix V stem bind L18 efficiently, even though that portion of 5S RNA is outside the L18 footprint. The L18 binding site is clearly more than a simple hairpin loop.

MeSH Terms

Base Sequence Binding Sites Chromosome Deletion DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Ribosomal/genetics RNA, Ribosomal, 5S/genetics,metabolism Ribosomal Proteins/metabolism Transcription, Genetic

Chemicals

RNA, Ribosomal RNA, Ribosomal, 5S Ribosomal Proteins ribosomal protein L18 DNA-Directed RNA Polymerases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gewirth D T

Department of Molecular Biophysics, Yale University, New Haven, CT 06511.

Moore P B

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.22.10717

Published

1988-11-25

Pages

10717-32

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338935

Subset

Grants

NIGMS NIH HHS · GM 22778 · United States

External Links

PubMed source DOI: 10.1093/nar/16.22.10717 PMC Full Text View journal details

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Exploration of the L18 binding site on 5S RNA by deletion mutagenesis.

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