Home LiteratureArticle Details
PMID: 3060849 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specialized binary vector for plant transformation: expression of the Arabidopsis thaliana AHAS gene in Nicotiana tabacum.

Nucleic acids research ·Vol. 16 ·No. 22 ·1988-11-25 ·Pages 10765-82

Olszewski NE, Martin FB, Ausubel FM

Abstract

We constructed a cosmid vector, pOCA18, designed for transferring plant genomic libraries from Agrobacterium tumefaciens to plants. Clones from a genomic library of Arabidopsis thaliana DNA in pOCA 18 were propagated stably in both Escherichia coli and A. tumefaciens. Clones from the pOCA18 A. thaliana library were used to construct transgenic Nicotiana tabacum plants; the DNA inserts were transferred intact in 10 out of 16 transgenic N. tabacum plants examined but were partially deleted in six others. Transgenic N. tabacum plants constructed with a mutant A. thaliana acetohydroxy acid synthase gene (from the pOCA18 library) that encodes an enzyme resistant to the herbicide chlorsulfuron were resistant to chlorsulfuron. A statistical analysis indicated that if the A. thaliana library contains 10(7) members and if 10(7) A. tumefaciens transconjugants containing the library were used to transform plant cells, then 2 x 10(4) transformed plant cells must be generated to have a 95% probability of constructing a transgenic plant carrying a specific DNA sequence from the A. thaliana library.

MeSH Terms
Cloning, Molecular Cosmids DNA/genetics,isolation & purification Drug Resistance/genetics Escherichia coli/genetics Genetic Vectors Herbicides/pharmacology Plants/drug effects,genetics Plants, Toxic Sulfonamides Tobacco/genetics Transformation, Genetic Triazines/pharmacology
Chemicals
Herbicides Sulfonamides Triazines DNA chlorsulfuron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olszewski N E
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Martin F B
Ausubel F M
References (26)
26 references, click to expand
  1. Utility of ethidium bromide in the extraction from whole plants of high molecular weight maize DNA.
    Plant Physiol. 1980 Dec;66(6):1140-3 PMID: 16661592
  2. A transcriptionally active, covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves.
    Cell. 1982 Jun;29(2):395-402 PMID: 7116445
  3. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  4. Octopine Ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the T-region.
    Plasmid. 1982 Jan;7(1):15-29 PMID: 6283573
  5. Chimeric genes as dominant selectable markers in plant cells.
    EMBO J. 1983;2(6):987-95 PMID: 16453464
  6. Introduction of genetic material into plant cells.
    Science. 1983 Nov 18;222(4625):815-21 PMID: 17738341
  7. The right hand copy of the nopaline Ti-plasmid 25 bp repeat is required for tumour formation.
    Nucleic Acids Res. 1984 Aug 10;12(15):6031-41 PMID: 6473100
  8. Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria.
    J Bacteriol. 1985 Oct;164(1):446-55 PMID: 4044529
  9. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.
    J Bacteriol. 1986 Dec;168(3):1291-301 PMID: 3782037
  10. Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4379-83 PMID: 2955406
  11. New cloning vehicles for transformation of higher plants.
    EMBO J. 1985 Feb;4(2):277-84 PMID: 16453603
  12. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  13. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens.
    EMBO J. 1986 Jul;5(7):1445-54 PMID: 3017694
  14. Agrobacterium-Mediated Gene Transfer Results Mainly in Transgenic Plants Transmitting T-DNA as a Single Mendelian Factor.
    Genetics. 1986 Sep;114(1):303-13 PMID: 17246346
  15. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.
    Science. 1986 Oct 24;234(4775):464-6 PMID: 17792019
  16. Binary Agrobacterium vectors for plant transformation.
    Nucleic Acids Res. 1984 Nov 26;12(22):8711-21 PMID: 6095209
  17. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  18. A binary vector for transferring genomic libraries to plants.
    Nucleic Acids Res. 1986 Oct 24;14(20):8073-90 PMID: 3534794
  19. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  20. Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4428-32 PMID: 16593716
  21. Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from agrobacterium to the plant genome.
    Cell. 1984 Sep;38(2):455-62 PMID: 6467373
  22. Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.
    J Bacteriol. 1983 Feb;153(2):878-83 PMID: 6296058
  23. Tumor DNA structure in plant cells transformed by A. tumefaciens.
    Science. 1980 Sep 19;209(4463):1385-91 PMID: 6251546
  24. The right border region of pTiT37 T-DNA is intrinsically more active than the left border region in promoting T-DNA transformation.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3895-9 PMID: 3459162
  25. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  26. Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid.
    EMBO J. 1986 Jun;5(6):1137-42 PMID: 15966101
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.22.10765
Published
1988-11-25
Pages
10765-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338938
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.22.10765 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2