The assembly of the major outer membrane protein OmpF of Escherichia coli depends on lipid synthesis.
Cerulenin, a drug which specifically blocks lipid synthesis, prevented both the trimerization of OmpF monomers and their assembly into the outer membrane of Escherichia coli B cells. A monoclonal antibody directed against a surface-exposed epitope of the trimer was used to probe the assembly of OmpF in the presence or absence of the drug. An inhibition level of 80% was reached 16 min after the addition of cerulenin. The accumulated monomeric form could not be assembled even after lipid synthesis was restored. Instead, it was slowly degraded. It was further shown that the inhibition of assembly resulted in a rapid inhibition of OmpF synthesis. These data demonstrate that there is a direct relationship between the synthesis of lipid (most likely lipopolysaccharide) and the correct export of OmpF. This coupling is required to promote the trimerization of the porin monomer and its assembly into the outer membrane.
29 references, click to expand
-
Phospholipid synthesis-dependent activity of aminopeptidase N in intact cells of Escherichia coli.J Mol Biol. 1979 Feb 25;128(2):127-41 PMID: 107320
-
Outer membrane of gram-negative bacteria. XVIII. Electron microscopic studies on porin insertion sites and growth of cell surface of Salmonella typhimurium.J Bacteriol. 1978 Aug;135(2):687-702 PMID: 355240
-
Regulation of the synthesis of surface protein in the cell cycle of E. coli B/r.Cell. 1979 Oct;18(2):287-96 PMID: 387259
-
Intermediate location in the assembly of the matrix protein or porin into the outer membrane of Escherichia coli.J Bacteriol. 1980 Sep;143(3):1538-41 PMID: 6997280
-
Precursor proteins are intermediates in vivo in the synthesis of two major outer membrane proteins, the OmpA and OmpF proteins, of Escherichia coli K12.Eur J Biochem. 1981 Jan;113(2):375-80 PMID: 7009158
-
Lipid-synthesis-dependent biosynthesis (or assembly) of major outer-membrane proteins of Escherichia coli.Eur J Biochem. 1981 Aug;118(1):105-11 PMID: 7026237
-
The receptor of bacteriophage lambda: evidence for a biosynthesis dependent on lipid synthesis.Eur J Biochem. 1982 Feb;122(2):381-6 PMID: 6460624
-
Subcellular distribution of various proteases in Escherichia coli.J Bacteriol. 1982 Mar;149(3):1027-33 PMID: 7037737
-
Maturation of exported proteins in Escherichia coli. Requirement for energy, site and kinetics of processing.Eur J Biochem. 1982 Jun;124(3):561-6 PMID: 6286306
-
Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria.Biochim Biophys Acta. 1983 Mar 21;737(1):51-115 PMID: 6337630
-
An intermediate step in translocation of lipopolysaccharide to the outer membrane of Salmonella typhimurium.Proc Natl Acad Sci U S A. 1983 Mar;80(5):1159-63 PMID: 6338498
-
Monoclonal antibodies reveal lamB antigenic determinants on both faces of the Escherichia coli outer membrane.J Bacteriol. 1983 Sep;155(3):1382-92 PMID: 6193098
-
Assembly pathway of newly synthesized LamB protein an outer membrane protein of Escherichia coli K-12.J Mol Biol. 1984 Jun 5;175(4):511-28 PMID: 6204059
-
Export of protein in bacteria.Microbiol Rev. 1984 Dec;48(4):290-8 PMID: 6394976
-
Molecular basis of bacterial outer membrane permeability.Microbiol Rev. 1985 Mar;49(1):1-32 PMID: 2580220
-
Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing attachment sites between the inner and outer membranes and the murein skeleton of the cell envelope.J Biol Chem. 1986 Jan 5;261(1):428-43 PMID: 3510202
-
The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.EMBO J. 1985 Dec 16;4(13A):3593-8 PMID: 3912172
-
An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export.J Biol Chem. 1986 Aug 25;261(24):11355-61 PMID: 2426271
-
Lack of effect of leader peptidase overproduction on the processing in vivo of exported proteins in Escherichia coli.J Gen Microbiol. 1986 Mar;132(3):689-96 PMID: 3525741
-
Periplasmic accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12.J Mol Biol. 1986 Jun 5;189(3):449-55 PMID: 3537309
-
Lipopolysaccharide tightly bound to porin monomers and trimers from Escherichia coli K-12.J Bacteriol. 1987 Sep;169(9):4003-10 PMID: 2442135
-
Export of protein in Escherichia coli: a novel mutation in ompC affects expression of other major outer membrane proteins.J Bacteriol. 1987 Sep;169(9):4327-34 PMID: 3305483
-
Immunological comparison of major outer membrane proteins from different strains of Escherichia coli.Ann Inst Pasteur Microbiol. 1987 Jul-Aug;138(4):393-406 PMID: 3311086
-
Genetics and biochemistry of the assembly of proteins into the outer membrane of E. coli.Prog Biophys Mol Biol. 1987;49(2-3):89-115 PMID: 3327100
-
A progenitor of the outer membrane LamB trimer.J Bacteriol. 1988 Apr;170(4):1973-4 PMID: 3280555
-
Immunological evidence for differences in the exposed regions of OmpF porins from Escherichia coli B and K-12.Mol Immunol. 1988 Jun;25(6):555-63 PMID: 2459611
-
Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.J Biol Chem. 1974 Dec 25;249(24):8019-29 PMID: 4609976
-
Size of the lipid precursor pool in Escherichia coli.J Bacteriol. 1978 Feb;133(2):1038 PMID: 342489
-
Lipid fluidity-dependent biosynthesis and assembly of the outer membrane proteins of E. coli.Cell. 1979 May;17(1):155-61 PMID: 378402
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
