Home LiteratureArticle Details
PMID: 3062363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4634-41

Siddiqui AH, Brandriss MC

Abstract

Deletion analysis of the promoter of the PUT2 gene that functions in the proline utilization pathway of Saccharomyces cerevisiae identified a PUT2 upstream activation site (UAS). It is contained within a single 40-base-pair (bp) region located immediately upstream of the TATA box and is both necessary and sufficient for proline induction. When placed upstream of a CYC7-lacZ gene fusion, the 40-bp sequence conferred proline regulation on CYC7-lacZ. A 35-bp deletion within the PUT2 UAS in an otherwise intact PUT2 promoter resulted in noninducible expression of a PUT2-lacZ gene fusion. When a plasmid bearing this UAS-deleted promoter was placed in a strain carrying a constitutive mutation in the positive regulatory gene PUT3, expression of PUT2-lacZ was not constitutive but occurred at levels below those found under noninducing conditions. In heterologous as well as homologous gene fusions, the PUT2 UAS appeared to be responsible for uninduced as well as proline-induced levels of expression. Although located immediately adjacent to the PUT2 UAS, the TATA box did not appear to play a regulatory role, as indicated by the results of experiments in which it was replaced by the CYC7 TATA box. A 26-bp sequence containing this TATA box was critical to the expression of PUT2, since a deletion of this region completely abolished transcriptional activity of the gene under both inducing and noninducing conditions. Our results indicate that the PUT2 promoter has a comparatively simple structure, requiring UAS and TATA sequences as well as the PUT3 gene product (directly or indirectly) for its expression.

MeSH Terms
1-Pyrroline-5-Carboxylate Dehydrogenase Base Sequence Chromosome Deletion DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Molecular Sequence Data Proline/metabolism Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
DNA, Fungal Saccharomyces cerevisiae Proteins Proline 1-Pyrroline-5-Carboxylate Dehydrogenase PUT2 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siddiqui A H
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2757.
Brandriss M C
References (64)
64 references, click to expand
  1. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.
    Cell. 1983 Jan;32(1):89-98 PMID: 6337724
  2. Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.
    Cell. 1982 Dec;31(2 Pt 1):319-25 PMID: 6297759
  3. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  4. Role of an upstream regulatory element in leucine repression of the Saccharomyces cerevisiae leu2 gene.
    Nature. 1984 Feb 23-29;307(5953):740-2 PMID: 6321998
  5. Misincorporation during DNA synthesis, analyzed by gel electrophoresis.
    Nucleic Acids Res. 1984 Apr 11;12(7):3155-71 PMID: 6326053
  6. MAL6 of Saccharomyces: a complex genetic locus containing three genes required for maltose fermentation.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2811-5 PMID: 6371820
  7. Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Oct;4(10):1985-98 PMID: 6390181
  8. A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.
    Mol Cell Biol. 1984 Oct;4(10):2023-30 PMID: 6095036
  9. A synthetic HIS4 regulatory element confers general amino acid control on the cytochrome c gene (CYC1) of yeast.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):498-502 PMID: 2982161
  10. Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2750-7 PMID: 6396505
  11. Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2837-42 PMID: 6098824
  12. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  13. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  14. Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer.
    Cell. 1985 May;41(1):41-8 PMID: 3888409
  15. Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase.
    Nucleic Acids Res. 1985 Jun 11;13(11):4011-27 PMID: 2989783
  16. A repressor (MAT alpha 2 Product) and its operator control expression of a set of cell type specific genes in yeast.
    Cell. 1985 Aug;42(1):237-47 PMID: 3893743
  17. Deletion analysis identifies a region, upstream of the ADH2 gene of Saccharomyces cerevisiae, which is required for ADR1-mediated derepression.
    Mol Cell Biol. 1985 Jul;5(7):1743-9 PMID: 3160930
  18. GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast.
    Cell. 1985 Nov;43(1):177-88 PMID: 3907851
  19. The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8557-61 PMID: 3909147
  20. Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562-6 PMID: 3001709
  21. Heme control region of the catalase T gene of the yeast Saccharomyces cerevisiae.
    Mol Gen Genet. 1986 Apr;203(1):73-8 PMID: 2423850
  22. General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene.
    Mol Cell Biol. 1985 Nov;5(11):3139-48 PMID: 3915770
  23. Saturation mutagenesis of the yeast his3 regulatory site: requirements for transcriptional induction and for binding by GCN4 activator protein.
    Science. 1986 Oct 24;234(4775):451-7 PMID: 3532321
  24. GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8516-20 PMID: 3464968
  25. Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes.
    Mol Cell Biol. 1986 Feb;6(2):674-87 PMID: 3023862
  26. Tandemly duplicated upstream control sequences mediate copper-induced transcription of the Saccharomyces cerevisiae copper-metallothionein gene.
    Mol Cell Biol. 1986 Apr;6(4):1158-63 PMID: 3537699
  27. Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2.
    Mol Cell Biol. 1986 Jul;6(7):2287-97 PMID: 3537717
  28. Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT1 gene.
    Mol Cell Biol. 1986 Jul;6(7):2638-45 PMID: 3537723
  29. Amino-terminal fragments of delta 1-pyrroline-5-carboxylate dehydrogenase direct beta-galactosidase to the mitochondrial matrix in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Oct;6(10):3502-12 PMID: 3025596
  30. Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.
    Mol Cell Biol. 1986 Dec;6(12):4335-43 PMID: 3540610
  31. A single Saccharomyces cerevisiae upstream activation site (UAS1) has two distinct regions essential for its activity.
    Mol Cell Biol. 1986 Dec;6(12):4690-6 PMID: 3025665
  32. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions.
    Gene. 1986;45(3):299-310 PMID: 3026915
  33. The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1340-4 PMID: 2881299
  34. Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene.
    Cell. 1987 Apr 10;49(1):9-18 PMID: 3030567
  35. Two related regulatory sequences are required for maximal induction of Saccharomyces cerevisiae his3 transcription.
    Mol Cell Biol. 1987 Jan;7(1):104-10 PMID: 3031449
  36. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  37. Ubiquitous upstream repression sequences control activation of the inducible arginase gene in yeast.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):3997-4001 PMID: 3295874
  38. Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene.
    Mol Cell Biol. 1987 May;7(5):1764-75 PMID: 3037334
  39. Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene.
    Mol Cell Biol. 1987 Jun;7(6):2212-20 PMID: 3037351
  40. A yeast operator overlaps an upstream activation site.
    Cell. 1987 Jul 31;50(3):369-77 PMID: 3301002
  41. LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.
    Mol Cell Biol. 1987 Aug;7(8):2708-17 PMID: 2823102
  42. Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences.
    Mol Cell Biol. 1987 Aug;7(8):2753-61 PMID: 3313003
  43. Identification of sequence elements that confer cell-type-specific control of MF alpha 1 expression in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Sep;7(9):3185-93 PMID: 2959859
  44. GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA.
    EMBO J. 1987 Sep;6(9):2781-4 PMID: 3678204
  45. Sequences required for transcriptional initiation of the Saccharomyces cerevisiae CYC7 genes.
    Mol Cell Biol. 1987 Oct;7(10):3785-91 PMID: 3316987
  46. Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner.
    Cell. 1987 Dec 24;51(6):953-61 PMID: 2826015
  47. Evidence for positive regulation of the proline utilization pathway in Saccharomyces cerevisiae.
    Genetics. 1987 Nov;117(3):429-35 PMID: 3121434
  48. Identification of a DNA segment that is necessary and sufficient for alpha-specific gene control in Saccharomyces cerevisiae: implications for regulation of alpha-specific and a-specific genes.
    Mol Cell Biol. 1988 Jan;8(1):309-20 PMID: 3275872
  49. Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product.
    Mol Cell Biol. 1987 Dec;7(12):4431-40 PMID: 3125423
  50. Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.
    Mol Cell Biol. 1988 Feb;8(2):647-54 PMID: 2832731
  51. ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance.
    Mol Cell Biol. 1988 Feb;8(2):664-73 PMID: 3280970
  52. Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae.
    Yeast. 1985 Dec;1(2):83-138 PMID: 3916863
  53. Transcription of adenovirus type 2 genes in a cell-free system: apparent heterogeneity of initiation at some promoters.
    Mol Cell Biol. 1981 Jul;1(7):635-51 PMID: 9279377
  54. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: mutation causing constitutive enzyme expression.
    J Bacteriol. 1979 Nov;140(2):504-7 PMID: 387738
  55. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  56. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  57. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  58. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  59. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  60. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  61. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  62. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline.
    J Bacteriol. 1979 Nov;140(2):498-503 PMID: 387737
  63. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  64. Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene.
    Mol Cell Biol. 1983 Oct;3(10):1846-56 PMID: 6358862
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4634-4641.1988
Published
1988-11-00
Pages
4634-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365552
Subset
IM
Databases
GENBANK
M22785
External Links
PubMed source DOI: 10.1128/mcb.8.11.4634-4641.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2