Home LiteratureArticle Details

PMID: 3063606 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing the HinfI restriction and modification genes.

Gene ·Vol. 70 ·No. 2 ·1988-10-30 ·Pages 387-92

Chandrasegaran S, Lunnen KD, Smith HO, Wilson GG

Abstract

The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Bacterial Deoxyribonucleases, Type II Site-Specific/genetics Escherichia coli/genetics Genes, Bacterial Haemophilus influenzae/genetics Molecular Sequence Data Plasmids Restriction Mapping

Chemicals

DNA, Bacterial Deoxyribonucleases, Type II Site-Specific GANTC-specific type II deoxyribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chandrasegaran S

Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205.

Lunnen K D

Smith H O

Wilson G G

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(88)90210-7

Published

1988-10-30

Pages

387-92

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

M22862

External Links

PubMed source DOI: 10.1016/0378-1119(88)90210-7 View journal details

Analysis Services

Cloning and sequencing the HinfI restriction and modification genes.

Contact

WeChat Official Account