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PMID: 3065609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Release factor competition is equivalent at strong and weakly suppressed nonsense codons.

Molecular & general genetics : MGG ·Vol. 213 ·No. 1 ·1988-07-00 ·Pages 144-9

Martin R, Hearn M, Jenny P, Gallant J

Abstract

We have compared the competition between strong or weak suppressor tRNAs and translational release factors (RF) at nonsense codons in the lacI gene of Escherichia coli. Using the F'lacIZ fusions developed by Miller and coworkers, UAG, UAA, and UGA codons at positions 189 and 220 were efficiently suppressed by plasmid-borne tRNA(trp) suppressors cognate to each nonsense triplet. Introduction of a compatible RF 1 plasmid competed at UAG and UAA but not UGA codons. An RF2 expressing plasmid competed at UAA and UGA but had little effect at UAG. Release factor competition against weak suppressors was measured using combinations of noncognate suppressors and nonsense codons. In each case, release factor plasmids behaved identically towards poorly suppressed codons as they did when the same codons were efficiently suppressed. The implications for these studies on the role of release factors in nonsense suppression context effects are discussed.

MeSH Terms
Codon Escherichia coli/genetics Escherichia coli Proteins Genes, Bacterial Peptide Termination Factors/metabolism Plasmids RNA, Messenger RNA, Transfer/genetics Suppression, Genetic
Chemicals
Codon Escherichia coli Proteins Peptide Termination Factors RNA, Messenger prfB protein, E coli RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Martin R
Department of Genetics, University of Washington, Seattle 98195.
Hearn M
Jenny P
Gallant J
References (21)
21 references, click to expand
  1. Peptide chain termination, codon, protein factor, and ribosomal requirements.
    Cold Spring Harb Symp Quant Biol. 1969;34:479-88 PMID: 4909515
  2. Genetic screen for cloned release factor genes.
    J Bacteriol. 1984 Apr;158(1):362-4 PMID: 6201475
  3. Cloning of the Escherichia coli release factor 2 gene.
    J Bacteriol. 1984 Apr;158(1):365-8 PMID: 6370964
  4. The energy relay: a proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5248-52 PMID: 6933556
  5. Peptide chain termination.
    Adv Protein Chem. 1973;27:243-76 PMID: 4589666
  6. Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs.
    J Bacteriol. 1984 Jun;158(3):849-59 PMID: 6327642
  7. Translation of the UGA triplet in vitro by tryptophan transfer RNA's.
    J Mol Biol. 1971 Jun 14;58(2):459-68 PMID: 4933413
  8. Characterization of three proteins involved in polypeptide chain termination.
    Cold Spring Harb Symp Quant Biol. 1969;34:469-77 PMID: 4909514
  9. Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.
    J Mol Biol. 1983 Feb 15;164(1):73-87 PMID: 6188841
  10. MSI and MSII made on ribosome in idling step of protein synthesis.
    Nature. 1972 Aug 18;238(5364):381-4 PMID: 4559580
  11. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  12. Nonsense suppression in aminoacyl-t-RNA limited cells.
    Mol Gen Genet. 1982;186(2):221-7 PMID: 6180282
  13. A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors.
    Mol Gen Genet. 1984;193(1):38-45 PMID: 6419024
  14. Release factors differing in specificity for terminator codons.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):768-74 PMID: 4879404
  15. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  16. Nonsense suppression context effects in Escherichia coli bacteriophage T4.
    Mol Gen Genet. 1987 May;207(2-3):517-8 PMID: 3302602
  17. Ribosomal mutations affecting efficiency of amber suppression.
    J Mol Biol. 1970 Feb 14;47(3):517-30 PMID: 4907270
  18. Translation rates and misreading characteristics of rpsD mutants in Escherichia coli.
    Mol Gen Genet. 1982;187(3):467-72 PMID: 6757661
  19. Mutants of the elongation factor EF-Tu, a new class of nonsense suppressors.
    EMBO J. 1985 Apr;4(4):1049-52 PMID: 3926487
  20. The context effect does not require a fourth base pair.
    Science. 1986 Jan 24;231(4736):393-5 PMID: 3510456
  21. Effects of surrounding sequence on the suppression of nonsense codons.
    J Mol Biol. 1983 Feb 15;164(1):59-71 PMID: 6188840
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333411
Published
1988-07-00
Pages
144-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM1362 · United States
NIGMS NIH HHS · GMO7735 · United States
External Links
PubMed source DOI: 10.1007/BF00333411
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