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PMID: 3069860 Published · ppublish English Journal Article

Cloned polynucleotide and synthetic oligonucleotide probes used in colony hybridization are equally efficient in the identification of enterotoxigenic Escherichia coli.

Journal of clinical microbiology ·Vol. 26 ·No. 11 ·1988-11-00 ·Pages 2275-8

Sommerfelt H, Kalland KH, Raj P, Moseley SL, Bhan MK, Bjorvatn B

Abstract

Restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic Escherichia coli. To compare their relative efficiencies, these two sets of probes were radiolabeled with 32P and were applied to 74 strains of E. coli with known enterotoxin profiles and to 156 previously unexamined E. coli isolates. The enterotoxigenic bacteria Vibrio cholerae O1, Vibrio cholerae non-O1 (NAG), Yersinia enterocolitica, and E. coli harboring the plasmid vectors of the polynucleotide gene probes were examined for further evaluation of probe specificity. The two classes of probes showed a perfect concordance in their specific detection and differentiation of enterotoxigenic E. coli. In the analysis of six strains, the signal strength on autoradiography after hybridization with oligonucleotides was weaker than that obtained after hybridization with polynucleotide probes. The probes did not hybridize with DNA from V. cholerae O1, V. cholerae non-O1 (NAG), or Y. enterocolitica. The strains of E. coli harboring the plasmid vectors of the polynucleotide gene probes were, likewise, negative in the hybridization assays.

MeSH Terms
Base Sequence DNA Probes Enterotoxins/genetics Escherichia coli/genetics,pathogenicity Genes Genes, Bacterial Nucleic Acid Hybridization Oligonucleotide Probes Phosphorus Radioisotopes Radioisotope Dilution Technique
Chemicals
DNA Probes Enterotoxins Oligonucleotide Probes Phosphorus Radioisotopes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sommerfelt H
Institute of International Health, University of Bergen, Norway.
Kalland K H
Raj P
Moseley S L
Bhan M K
Bjorvatn B
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1988-11-00
Pages
2275-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC266875
Subset
IM
Corrections
ErratumIn
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