Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.

Gene ·Vol. 74 ·No. 1 ·1988-12-25 ·Pages 55-9

Van Cott EM, Wilson GG

Abstract

Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.

MeSH Terms

Cloning, Molecular DNA-Cytosine Methylases/genetics Deoxyribonucleases, Type II Site-Specific/genetics Escherichia coli/genetics Fusobacterium/enzymology,genetics Genes Genes, Bacterial Nocardia/enzymology,genetics Recombinant Proteins/genetics Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics Xanthomonas/enzymology,genetics

Chemicals

Recombinant Proteins DNA modification methylase NaeI DNA modification methylase NcoI DNA modification methylase XbaI DNA-Cytosine Methylases Cytosine 5-methyltransferase Site-Specific DNA-Methyltransferase (Adenine-Specific) endodeoxyribonuclease XBAI Deoxyribonucleases, Type II Site-Specific GGCC-specific type II deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Van Cott E M

New England Biolabs, Inc., Beverly, MA 01915.

Wilson G G

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(88)90251-x

Published

1988-12-25

Pages

55-9

Language

English

Region

Netherlands

NLM ID

7706761

Subset

External Links

PubMed source DOI: 10.1016/0378-1119(88)90251-x View journal details

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Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems.

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