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PMID: 3080405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3.

Journal of bacteriology ·Vol. 165 ·No. 2 ·1986-02-00 ·Pages 448-52

Cavenagh MM, Miller RV

Abstract

D3, a temperate bacteriophage of Pseudomonas aeruginosa PAO, was found to specifically transduce the alleles met-49 and met-117. Induction of established lysogens with UV light was necessary for the production of transducing lysates. Transduced cells were immune to superinfection by phage D3 and could give rise to high-frequency transducing lysates. Cotransduction of these two alleles could not be demonstrated. met-117 was mapped to 26 min on the PAO genetic map. Complementation studies using the generalized transducing phage F116L indicated that met-49 is an allele of met-9011 which maps at 55 min. The integrated D3 prophage was shown to be coinherited with met-117 and with met-49.

MeSH Terms
Bacteriophages/genetics Chromosome Mapping Chromosomes, Bacterial Genes, Bacterial Methionine/genetics Pseudomonas aeruginosa/genetics Recombination, Genetic Transduction, Genetic
Chemicals
Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cavenagh M M
Miller R V
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.2.448-452.1986
Published
1986-02-00
Pages
448-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214439
Subset
IM
Grants
NIAID NIH HHS · AI-12759 · United States
External Links
PubMed source DOI: 10.1128/jb.165.2.448-452.1986 PMC Full Text View journal details
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