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PMID: 3081305 Published · ppublish English Journal Article

Chromosome distribution of intracisternal A-particle sequences in the Syrian hamster and mouse.

Chromosoma ·Vol. 93 ·No. 3 ·1986-00-00 ·Pages 213-9

Kuff EL, Fewell JE, Lueders KK, DiPaolo JA, Amsbaugh SC, Popescu NC

Abstract

Metaphase chromosomes of Syrian hamster and BALB/c mice were hybridized in situ with radiolabeled probes derived from cloned intracisternal A-particle (IAP) genes of the corresponding species. The DNAs of these species are known to contain about 900 and 1,000 copies, respectively, of the retrovirus-like IAP sequence elements per haploid genome. Multiple IAP sequences were found on all chromosomes of both hamster and mouse. In the hamster, more than half of the IAP sequences were located in regions of non-centromeric constitutive heterochromatin, at an average concentration per unit chromosome length 5 times greater than in the euchromatic regions. The other dispersed sequences showed marked local variations in concentration along the chromosome lengths; both discrete foci and large grain clusters were observed as well as regions apparently lacking IAP sequences. Within the resolution of the techniques, IAP sequences appeared to be more evenly distributed over the mouse chromosomes; however, some prominent variations in concentration were seen. The number of potentially active IAP genes in the Syrian hamster, and by extension in the mouse, may be restricted by the preferential location of IAP sequences in genetically inert regions of the genome.

MeSH Terms
Animals Base Sequence Chromosomes/ultrastructure Cricetinae Embryo, Mammalian Female Fetus Genes, Intracisternal A-Particle Karyotyping Male Mesocricetus Metaphase Mice Mice, Inbred BALB C Nucleic Acid Hybridization Pregnancy Proto-Oncogenes Skin/cytology Species Specificity
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kuff E L
Fewell J E
Lueders K K
DiPaolo J A
Amsbaugh S C
Popescu N C
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41 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292740
Published
1986-00-00
Pages
213-9
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292740 View journal details
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