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PMID: 3081488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of cis-acting regulatory alleles of the Bacillus subtilis amyR region by using gene conversion transformation.

Journal of bacteriology ·Vol. 165 ·No. 3 ·1986-03-00 ·Pages 663-70

Nicholson WL, Chambliss GH

Abstract

Three cis-acting alleles (gra-10, gra-5, and amyR2) of the Bacillus subtilis amyR promoter locus each cause catabolite repression-resistance of amyE-encoded alpha-amylase synthesis. The gra-10, gra-5, and amyR2 alleles were transferred from the chromosomes of their respective hosts to a plasmid carrying the amyR1-amyE+ gene by the process of gene conversion which is carried out during transformation of competent B. subtilis by plasmid clones carrying homologous DNA. The cloned amyR promoter regions containing the gra-10 and gra-5 mutations were shown to confer catabolite repression-resistance in cis to the synthesis of chloramphenicol acetyltransferase encoded by the cat-86 indicator gene when subcloned into the promoter-probe plasmid pPL603B. Implications concerning both the regulation of amyR utilization and the process of gene conversion in B. subtilis are discussed.

MeSH Terms
Alleles Bacillus subtilis/enzymology,genetics Cloning, Molecular Enzyme Repression Gene Conversion Genes, Bacterial Glucose/pharmacology Mutation Phenotype Plasmids Promoter Regions, Genetic Transformation, Bacterial alpha-Amylases/biosynthesis,genetics
Chemicals
alpha-Amylases Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nicholson W L
Chambliss G H
References (32)
32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.3.663-670.1986
Published
1986-03-00
Pages
663-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214480
Subset
IM
Grants
NIGMS NIH HHS · GM 07133 · United States
NIGMS NIH HHS · GM 32199 · United States
NIGMS NIH HHS · GM 34324 · United States
External Links
PubMed source DOI: 10.1128/jb.165.3.663-670.1986 PMC Full Text View journal details
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