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PMID: 3086221 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The cellular pathway of antigen presentation: biochemical and functional analysis of antigen processing in dendritic cells and macrophages.

Immunology ·Vol. 58 ·No. 2 ·1986-06-00 ·Pages 271-6

Chain BM, Kay PM, Feldmann M

Abstract

The response of primed T cells to keyhole limpet haemocyanin (KLH) was used to compare the characteristics of antigen presentation by lymphoid dendritic cells, splenic and peritoneal macrophages. In a similar manner to macrophages, purified dendritic cells could be pulsed with antigen and subsequently fixed by brief glutaraldehyde fixation and still retain antigen presenting activity. Also, as previously reported for macrophages, presentation could be inhibited by chloroquine. These functional experiments suggested that the pathway of antigen presentation in dendritic cells and macrophages was similar or identical. However, biochemical studies, using radiolabelled antigen, showed that dendritic cells do not significantly degrade large proteins such as KLH to TCA-soluble form, but partially hydrolyse them to smaller peptide fragments. The significance of these results in terms of a model of the cellular pathways of antigen presentation is discussed.

MeSH Terms
Animals Antigen-Presenting Cells/drug effects,immunology Antigens/immunology Chloroquine/pharmacology Dose-Response Relationship, Immunologic Glutaral Hemocyanins/metabolism Lymphoid Tissue/cytology,immunology,metabolism Macrophages/immunology Mice Mice, Inbred CBA Mitosis Receptors, Fc/analysis T-Lymphocytes/immunology
Chemicals
Antigens Receptors, Fc Chloroquine Hemocyanins keyhole-limpet hemocyanin Glutaral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chain B M
Kay P M
Feldmann M
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19 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1986-06-00
Pages
271-6
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1452653
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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