Home LiteratureArticle Details
PMID: 3092223 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Negative trans-regulation of T-cell antigen receptor/T3 complex mRNA expression in murine T-lymphoma somatic cell hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 18 ·1986-09-00 ·Pages 6989-93

MacLeod CL, Minning L, Gold DP, Terhorst C, Wilkinson M

Abstract

The antigen-specific T-cell receptor (TCR) is composed of variable antigen-recognition chains TCR-alpha and TCR-beta in noncovalent association with the invariant T3 multimer. The TCR-alpha and TCR-beta chains are encoded by gene segments that must be juxtaposed by rearrangement in order to be expressed. To examine whether mechanisms other than gene rearrangement might regulate TCR/T3 gene expression, somatic cell hybrids were formed among closely related murine SL12 T-lymphoma clones that differ in TCR/T3 mRNA levels. In hybrid cells formed between cell clones in which one parent is TCR-beta+ and the other is TCR-beta-, the resultant hybrid cells lack detectable TCR-beta transcripts. Since the protein synthesis inhibitor cycloheximide partially reverses TCR-beta repression in the hybrid cells, we postulate that a labile repressor protein is involved. The amount of mRNA encoding one of the T3 polypeptide chains, T3-delta, is also strongly negatively transregulated in the same hybrid cells in which TCR-beta mRNA expression is repressed. The negative trans-regulation of TCR-beta and T3-delta mRNA expression is relatively specific, since the levels of TCR-alpha mRNA and several thymocyte surface antigens are not repressed in somatic cell hybrids. Our results indicate that rearrangement of the TCR genes alone is not sufficient for TCR-beta expression and that trans-acting factors regulate the amounts of both TCR-beta and T3-delta mRNA in this system.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte Antigens, Surface/genetics Cell Line Cycloheximide/pharmacology Gene Expression Regulation Hybrid Cells Lymphoma/immunology Mice RNA, Messenger/analysis Receptors, Antigen, T-Cell/genetics Transcription, Genetic
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface RNA, Messenger Receptors, Antigen, T-Cell Cycloheximide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
MacLeod C L
Minning L
Gold D P
Terhorst C
Wilkinson M
References (31)
31 references, click to expand
  1. The T3 complex on human T lymphocytes involves four structurally distinct glycoproteins.
    J Biol Chem. 1983 Apr 25;258(8):5135-41 PMID: 6220014
  2. Assignment of the gene coding for the T3-delta subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2920-4 PMID: 3857625
  3. The mouse T cell receptor: comparison of MHC-restricted receptors on two T cell hybridomas.
    Cell. 1983 Oct;34(3):727-37 PMID: 6605198
  4. The mouse T cell receptor: structural heterogeneity of molecules of normal T cells defined by xenoantiserum.
    Cell. 1983 Oct;34(3):739-46 PMID: 6194888
  5. Monoclonal antibodies against the antigen receptor on a cloned T-cell hybrid.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6972-6 PMID: 6316339
  6. Expression of transferrin receptor on murine hematopoietic progenitors.
    Cell Immunol. 1984 Jan;83(1):14-25 PMID: 6319033
  7. Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.
    Nature. 1984 Mar 8-14;308(5955):149-53 PMID: 6199676
  8. A new murine model system for the in vitro development of thymoma cell heterogeneity.
    Cancer Res. 1984 May;44(5):1784-90 PMID: 6201262
  9. Hybridization of nucleic acids immobilized on solid supports.
    Anal Biochem. 1984 May 1;138(2):267-84 PMID: 6204550
  10. Thymocyte subpopulation enriched for progenitors with an unrearranged T-cell receptor beta-chain gene.
    Nature. 1985 Jun 20-26;315(6021):666-9 PMID: 3874369
  11. Expression of genes of the T-cell antigen receptor complex in precursor thymocytes.
    Nature. 1985 Jun 27-Jul 3;315(6022):765-8 PMID: 4010781
  12. The T cell receptor: the alpha and beta chains define idiotype, and antigen and MHC specificity.
    Cell. 1985 Aug;42(1):81-7 PMID: 2410143
  13. Genes encoding the T-cell receptor alpha and beta subunits are transcribed in an ordered manner during intrathymic ontogeny.
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5510-4 PMID: 2410928
  14. Genetic complexity of glucocorticoid-induced lysis of murine T-lymphoma cells.
    Cancer Res. 1985 Oct;45(10):4804-9 PMID: 3875400
  15. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer.
    Nature. 1985 Aug 15-21;316(6029):606-9 PMID: 4033759
  16. The human T-cell receptor.
    Annu Rev Immunol. 1984;2:23-50 PMID: 6443341
  17. Differentiation and cell division in the mammalian thymus.
    Dev Biol. 1985 Nov;112(1):1-17 PMID: 3932107
  18. Identification of the components of the murine T cell antigen receptor complex.
    Cell. 1985 Nov;43(1):223-31 PMID: 3878228
  19. A T3-like protein complex associated with the antigen receptor on murine T cells.
    Nature. 1986 Mar 20-26;320(6059):272-5 PMID: 2938011
  20. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition.
    Annu Rev Immunol. 1986;4:529-91 PMID: 3518750
  21. Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex.
    Nature. 1986 May 22-28;321(6068):431-4 PMID: 3012357
  22. Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex.
    Cell. 1986 Jul 4;46(1):75-87 PMID: 3087626
  23. Genomic organization and sequence of T-cell receptor beta-chain constant- and joining-region genes.
    Nature. 1984 Aug 2-8;310(5976):387-91 PMID: 6336329
  24. The structure, rearrangement and expression of D beta gene segments of the murine T-cell antigen receptor.
    Nature. 1984 Sep 27-Oct 3;311(5984):344-50 PMID: 6090932
  25. A third type of murine T-cell receptor gene.
    Nature. 1984 Nov 1-7;312(5989):31-5 PMID: 6548551
  26. Sequence and expression of transcripts of the human T-cell receptor beta-chain genes.
    Nature. 1984 Dec 6-12;312(5994):521-4 PMID: 6334237
  27. Ontogeny of the T-cell antigen receptor within the thymus.
    Nature. 1985 Feb 14-20;313(6003):592-5 PMID: 3871511
  28. Developmental regulation of T-cell receptor gene expression.
    Nature. 1985 Mar 7-13;314(6006):103-7 PMID: 2983227
  29. SL12 murine T-lymphoma: a new model for tumor cell heterogeneity.
    J Natl Cancer Inst. 1985 Apr;74(4):875-82 PMID: 3872958
  30. Isolation and characterization of a cDNA clone encoding the murine homologue of the human 20K T3/T-cell receptor glycoprotein.
    Nature. 1985 Apr 11-17;314(6011):542-4 PMID: 2859526
  31. A new determinant of glucocorticoid sensitivity in lymphoid cell lines.
    J Cell Biol. 1983 Feb;96(2):409-15 PMID: 6601107
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.18.6989
Published
1986-09-00
Pages
6989-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386637
Subset
IM
Grants
NIAID NIH HHS · AI17651 · United States
NCI NIH HHS · CA12386 · United States
NCI NIH HHS · CA37778 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.18.6989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2