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PMID: 3095371 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heating cells in acid methanol for 30 min without freeze-drying provides adequate yields of fatty acids and alcohols for gas chromatographic characterization of mycobacteria.

Journal of clinical microbiology ·Vol. 24 ·No. 5 ·1986-11-00 ·Pages 844-5

Jimenez J, Larsson L

Abstract

We studied the release of mycobacterial fatty acids (as methyl esters) and secondary alcohols after heating both wet and freeze-dried cells in methanolic hydrogen chloride for different time periods. A 30-min heating of the mycobacteria without prior freeze-drying was found adequate in a routine gas chromatographic procedure for strain and species characterization.

MeSH Terms

Alcohols/analysis Chromatography, Gas Fatty Acids/analysis Freeze Drying Hot Temperature Hydrochloric Acid Methanol Mycobacterium/analysis,classification Mycobacterium avium/analysis,classification Mycobacterium tuberculosis/analysis,classification

Chemicals

Alcohols Fatty Acids Hydrochloric Acid Methanol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jimenez J

Larsson L

References (4)

4 references, click to expand

Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone.
J Clin Microbiol. 1979 Oct;10(4):506-14 PMID: 118984
Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography.
J Clin Microbiol. 1986 Apr;23(4):731-6 PMID: 3084554
Influence of culture medium and incubation time on the fatty acid compositions of some rapid-growing mycobacteria as analysed by packed and capillary column gas chromatography.
Acta Pathol Microbiol Immunol Scand B. 1985 Oct;93(5):353-7 PMID: 4083012
Gas chromatographic fatty acid profiles for characterisation of mycobacteria: an interlaboratory methodological evaluation.
Eur J Clin Microbiol. 1985 Oct;4(5):483-7 PMID: 4065134

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.24.5.844-845.1986

Published

1986-11-00

Pages

844-5

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC269039

Subset

External Links

PubMed source DOI: 10.1128/jcm.24.5.844-845.1986 PMC Full Text View journal details

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Heating cells in acid methanol for 30 min without freeze-drying provides adequate yields of fatty acids and alcohols for gas chromatographic characterization of mycobacteria.

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