Home LiteratureArticle Details
PMID: 3096727 Published · ppublish English Journal Article

Modulation of adherence of coagulase-negative staphylococci to Teflon catheters in vitro.

European journal of clinical microbiology ·Vol. 5 ·No. 5 ·1986-10-00 ·Pages 518-22

Pascual A, Fleer A, Westerdaal NA, Verhoef J

Abstract

The mechanism of adherence of Staphylococcus epidermidis to commercially available catheters was studied in vitro in a quantitative assay employing 3H-labelled bacteria. It was found that adherence to Teflon catheters was significantly related to the degree of hydrophobicity of the strains. When hydrophobic groups were removed from Staphylococcus epidermidis by pepsin treatment, adhesion was almost completely abolished. Preincubation of catheters in human serum also caused a 80-90% reduction of adherence. Preincubation of Staphylococcus epidermidis in serum similarly decreased adhesion. This effect of serum was mainly due to albumin, while IgG and fibronectin were less effective. Culture of Staphylococcus epidermidis in subinhibitory concentrations (0.5 MIC) of cephalothin, clindamycin and vancomycin resulted in a 30-80% reduction in adhesion.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Adhesion Blood Proteins/pharmacology Catheters, Indwelling Coagulase Fibronectins/pharmacology Humans Immunoglobulin G/pharmacology Infant, Newborn Kinetics Pepsin A/pharmacology Polytetrafluoroethylene/metabolism Staphylococcal Infections/prevention & control Staphylococcus epidermidis/metabolism
Chemicals
Anti-Bacterial Agents Blood Proteins Coagulase Fibronectins Immunoglobulin G Polytetrafluoroethylene Pepsin A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pascual A
Fleer A
Westerdaal N A
Verhoef J
References (19)
19 references, click to expand
  1. Adherence of bacteria to pediatric intravenous catheters and needles and its relation to phlebitis in animals.
    Pediatr Res. 1984 Dec;18(12):1361-6 PMID: 6395081
  2. Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium.
    J Biol Chem. 1982 May 10;257(9):4888-93 PMID: 7068668
  3. Attachment of staphylococci to various synthetic polymers.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Apr;256(4):479-89 PMID: 6741344
  4. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
    J Clin Microbiol. 1985 Dec;22(6):996-1006 PMID: 3905855
  5. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces.
    Infect Immun. 1982 Jul;37(1):318-26 PMID: 6179880
  6. Changing blood culture isolates in a referral neonatal intensive care unit.
    Arch Dis Child. 1981 Oct;56(10):775-8 PMID: 7305416
  7. Bacterial adherence to fibronectin and endothelial cells: a possible mechanism for bacterial tissue tropism.
    J Lab Clin Med. 1984 Jan;103(1):34-43 PMID: 6361186
  8. Adsorption of fibronectin onto polymethylmethacrylate and promotion of Staphylococcus aureus adherence.
    Infect Immun. 1984 Sep;45(3):768-74 PMID: 6469360
  9. Quantitative adherence in vitro of coagulase-negative staphylococci to intravascular catheters: inhibition with D-mannosamine.
    J Infect Dis. 1984 Jan;149(1):116 PMID: 6693784
  10. Nosocomial septicemia due to multiply antibiotic-resistant Staphylococcus epidermidis.
    Ann Intern Med. 1982 Jan;96(1):1-10 PMID: 7053681
  11. Adhesion of coagulase-negative staphylococci to biomaterials.
    J Gen Microbiol. 1983 Sep;129(9):2959-68 PMID: 6631420
  12. Cell surface characteristics of coagulase-negative staphylococci and their adherence to fluorinated poly(ethylenepropylene).
    Infect Immun. 1986 Jan;51(1):294-301 PMID: 3940998
  13. Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters.
    J Infect Dis. 1982 Oct;146(4):479-82 PMID: 7119478
  14. Coagulase-negative staphylococcal septicemia: experience in a newborn intensive care unit.
    J Pediatr. 1982 Oct;101(4):602-5 PMID: 6811714
  15. Scanning and transmission electron microscopy of in situ bacterial colonization of intravenous and intraarterial catheters.
    J Clin Microbiol. 1984 May;19(5):687-93 PMID: 6429190
  16. Septicemia due to coagulase-negative staphylococci in a neonatal intensive care unit: clinical and bacteriological features and contaminated parenteral fluids as a source of sepsis.
    Pediatr Infect Dis. 1983 Nov-Dec;2(6):426-31 PMID: 6419206
  17. Simplified scheme for routine identification of human Staphylococcus species.
    J Clin Microbiol. 1975 Jan;1(1):82-8 PMID: 170303
  18. Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy.
    J Clin Microbiol. 1983 Dec;18(6):1388-98 PMID: 6228562
  19. Factors governing adherence of Candida species to plastic surfaces.
    Infect Immun. 1985 Oct;50(1):97-101 PMID: 3899942
Article Info
Journal
European journal of clinical microbiology
Abbr.
Eur J Clin Microbiol
ISSN
0722-2211
DOI
10.1007/BF02017694
Published
1986-10-00
Pages
518-22
Language
English
Region
Germany
NLM ID
8219582
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02017694
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2