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PMID: 3098784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Enhancement of natural killer function through activation of the T11 E rosette receptor.

The Journal of clinical investigation ·Vol. 79 ·No. 1 ·1987-01-00 ·Pages 305-8

Schmidt RE, Michon JM, Woronicz J, Schlossman SF, Reinherz EL, Ritz J

Abstract

Natural killer (NK) cells, which represent a small fraction of normal peripheral blood mononuclear cells, were purified by immunofluorescent cell sorting of NKH1+ cells. cytotoxicity of NKH1+ cells could be enhanced through activation by monoclonal antibodies (anti-T11(2) and anti-T11(3)) specific for epitopes of the sheep erythrocyte receptor or by recombinant interleukin-2 (rIL-2). After 18 h, incubation with both anti-T11(2/3) and rIL-2 resulted in similar levels of enhanced cytotoxicity against NK-resistant as well as NK-sensitive targets. Before and after induction, cytotoxicity was found predominantly within the NKH1+ population. These results suggest that several distinct mechanisms may be capable of enhancing NK activity and that the cells responsible for lymphokine-activated killing are likely to be the same population capable of spontaneous or natural killing before activation in vitro.

MeSH Terms

Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface/immunology Cell Line Cytotoxicity, Immunologic Immunity, Innate Killer Cells, Natural/immunology Receptors, Immunologic/immunology Rosette Formation

Chemicals

Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Surface Receptors, Immunologic

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Schmidt R E

Michon J M

Woronicz J

Schlossman S F

Reinherz E L

Ritz J

References (20)

20 references, click to expand

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI112800

Published

1987-01-00

Pages

305-8

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC424050

Subset

Grants

NCI NIH HHS · CA-41619 · United States

External Links

PubMed source DOI: 10.1172/JCI112800 PMC Full Text View journal details

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Enhancement of natural killer function through activation of the T11 E rosette receptor.

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