B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.

The Journal of experimental medicine ·Vol. 148 ·No. 6 ·1978-12-01 ·Pages 1612-9

Schreier MH

Abstract

A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.

MeSH Terms

Animals Antibody Formation B-Lymphocytes/cytology,immunology Cell Differentiation Cells, Cultured Dose-Response Relationship, Immunologic Erythrocytes/immunology Female Lymphocyte Cooperation Male Mice Mice, Inbred Strains Sheep T-Lymphocytes/immunology

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Schreier M H

References (11)

11 references, click to expand

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Precursor cells specific to sheep red cells in nude mice. Estimation of frequency in the microculture system.
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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.148.6.1612

Published

1978-12-01

Pages

1612-9

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2185108

Subset

External Links

PubMed source DOI: 10.1084/jem.148.6.1612 PMC Full Text View journal details

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B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.

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