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PMID: 3106978 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of a dispersed, multicopy, X chromosome sequence in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2843-7

Waring GL, Pollack JC

Abstract

We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A + T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila.

MeSH Terms
Animals Base Sequence Cloning, Molecular Drosophila melanogaster/genetics Nucleic Acid Hybridization Sequence Homology, Nucleic Acid X Chromosome
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Waring G L
Pollack J C
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2843
Published
1987-05-00
Pages
2843-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304756
Subset
IM
Grants
NICHD NIH HHS · HD00524 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.9.2843 PMC Full Text View journal details
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