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PMID: 3110115 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The binding of epidermolytic toxin from Staphylococcus aureus to mouse epidermal tissue.

The Histochemical journal ·Vol. 19 ·No. 3 ·1987-03-00 ·Pages 137-49

Smith TP, John DA, Bailey CJ

Abstract

Fluorescein-labelled epidermolytic toxin (FTC-toxin) of Staphylococcus aureus and ferritin-toxin conjugate have been prepared and purified. FTC-toxin bound selectively to cryostat and resin-impregnated sections of neonatal mouse skin. Binding was localized at the keratohyalin granules and in the stratum corneum. In an epidermal cell (granular, spinous and basal) preparation, only keratohyalin granules of the granular cells bound FTC-toxin. Ferritin-toxin conjugate bound to skin sections at the same two sites as FTC-toxin and was competitive with the binding of free toxin. Keratohyalin granules in unstained sections had a novel 'patched' appearance under the electron microscope, and the ferritin-toxin conjugate bound preferentially to the electron-lucent areas. In the stratum corneum it was shown by quantitative estimation that the target density decreased as the surface of the tissue was approached.

MeSH Terms

Animals Animals, Newborn Bacterial Toxins/metabolism Ferritins Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Antibody Technique Fluorescent Dyes Mice Microscopy, Electron Receptors, Immunologic/metabolism Skin/cytology,metabolism,ultrastructure Staphylococcus aureus Thiocyanates

Chemicals

Bacterial Toxins Fluoresceins Fluorescent Dyes Receptors, Immunologic Thiocyanates Ferritins Fluorescein-5-isothiocyanate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Smith T P

John D A

Bailey C J

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25 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01695138

Published

1987-03-00

Pages

137-49

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01695138

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The binding of epidermolytic toxin from Staphylococcus aureus to mouse epidermal tissue.

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