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PMID: 3114231 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 3969-75

Harms N, de Vries GE, Maurer K, Hoogendijk J, Stouthamer AH

Abstract

A genomic clone bank of Paracoccus denitrificans DNA has been constructed in the expression vector set pEX1, pEX2, and pEX3. Screening of this clone bank with antibodies raised against P. denitrificans methanol dehydrogenase resulted in the isolation of a clone, pNH3, that synthesized methanol dehydrogenase cross-reactive proteins. The nucleotide sequence of the P. denitrificans DNA fragment inserted in this clone has been determined and shown to contain the full methanol dehydrogenase structural gene. DNA cross-hybridization was found with DNA fragments which have been reported to contain the methanol dehydrogenase structural genes from Methylobacterium sp. strain AM1 and Methylobacterium organophilum.

MeSH Terms
Alcohol Oxidoreductases/genetics,immunology,isolation & purification Amino Acid Sequence Base Sequence Cloning, Molecular Codon Cross Reactions DNA, Bacterial/analysis Electrophoresis, Polyacrylamide Gel Genes Genes, Bacterial Genetic Vectors Immunoassay Immunodiffusion Nucleic Acid Hybridization Paracoccus denitrificans/enzymology,genetics Plasmids Promoter Regions, Genetic Sequence Homology, Nucleic Acid
Chemicals
Codon DNA, Bacterial Alcohol Oxidoreductases alcohol dehydrogenase (acceptor)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Harms N
de Vries G E
Maurer K
Hoogendijk J
Stouthamer A H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.9.3969-3975.1987
Published
1987-09-00
Pages
3969-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213695
Subset
IM
Databases
GENBANK
M17339
External Links
PubMed source DOI: 10.1128/jb.169.9.3969-3975.1987 PMC Full Text View journal details
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